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RNA interference

  • RESEARCH ARTICLE
    Prostaglandin catabolism in Spodoptera exigua, a lepidopteran insect
    Shabbir Ahmed, Yonggyun Kim
    Journal of Experimental Biology 2020 223: jeb233221 doi: 10.1242/jeb.233221 Published 4 November 2020

    Highlighted Article: Excessive levels of prostaglandins (PGs) are detrimental to animal survival and should be degraded; this study reports PG degradation with identification of genes encoding PG dehydrogenase (PGDH) and PG reductase (PGR) in a lepidopteran insect.

  • RESEARCH ARTICLE
    Electrical interactions between photoreceptors in the compound eye of Periplaneta americana
    Paulus Saari, Esa-Ville Immonen, Andrew S. French, Päivi H. Torkkeli, Hongxia Liu, Kyösti Heimonen, Roman V. Frolov
    Journal of Experimental Biology 2018 221: jeb189340 doi: 10.1242/jeb.189340 Published 1 November 2018

    Summary: In the compound eye of the American cockroach, complex electrical interactions take place between green- and UV-sensitive photoreceptors in each ommatidium.

  • RESEARCH ARTICLE
    ArHsp40 and ArHsp40-2 contribute to stress tolerance and longevity in Artemia franciscana, but only ArHsp40 influences diapause entry
    Nathan M. Rowarth, Thomas H. MacRae
    Journal of Experimental Biology 2018 221: jeb189001 doi: 10.1242/jeb.189001 Published 19 October 2018

    Summary: Two Hsp40s function differently from one another in diapause entry, stress tolerance and longevity of Artemia franciscana.

  • METHODS & TECHNIQUES
    Silencing cuticular pigmentation genes enables RNA FISH in intact insect appendages
    Stefan Pentzold, Veit Grabe, Andrei Ogonkov, Lydia Schmidt, Wilhelm Boland, Antje Burse
    Journal of Experimental Biology 2018 221: jeb185710 doi: 10.1242/jeb.185710 Published 25 September 2018

    Summary: Clearing cuticular pigmentation in beetles by gene silencing enables the use of intact appendages for fluorescence in situ hybridisation.

  • RESEARCH ARTICLE
    Reverse genetics demonstrate the role of mucosal C-type lectins in food particle selection in the oyster Crassostrea virginica
    Emmanuelle Pales Espinosa, Bassem Allam
    Journal of Experimental Biology 2018 221: jeb174094 doi: 10.1242/jeb.174094 Published 14 March 2018

    Summary: Carbohydrate–protein interactions enable efficient food particle sorting in bivalves, establishing a new dimension for the role of evolutionarily conserved mannose/glucose-binding proteins in the metazoan.

  • RESEARCH ARTICLE
    Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
    Marianna Pauletto, Amélie Segarra, Caroline Montagnani, Virgile Quillien, Nicole Faury, Jacqueline Le Grand, Philippe Miner, Bruno Petton, Yannick Labreuche, Elodie Fleury, Caroline Fabioux, Luca Bargelloni, Tristan Renault, Arnaud Huvet
    Journal of Experimental Biology 2017 220: 3671-3685; doi: 10.1242/jeb.156299

    Summary: Double-stranded ribonucleic acid (dsRNA) injection in the Pacific oyster induced an anti-viral state controlling ostreid herpesvirus 1 replication and precluding the understanding of the role of the inhibitor of nuclear factor-κB, Cg-IκB2.

  • METHODS & TECHNIQUES
    siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
    Gen Zhang, Li-Sheng He, Yue Him Wong, Li Yu, Pei-Yuan Qian
    Journal of Experimental Biology 2015 218: 2505-2509; doi: 10.1242/jeb.120113

    Summary: siRNA transfection using RNAfectin at both nauplius VI and cyprid stages is effective in knocking down gene expression in barnacles.

  • RESEARCH ARTICLES
    Functional circadian clock genes are essential for the overwintering diapause of the Northern house mosquito, Culex pipiens
    Megan E. Meuti, Mary Stone, Tomoko Ikeno, David L. Denlinger
    Journal of Experimental Biology 2015 218: 412-422; doi: 10.1242/jeb.113233
  • RESEARCH ARTICLES
    Involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bug, Riptortus pedestris
    Tomoko Ikeno, Hideharu Numata, Shin G. Goto, Sakiko Shiga
    Journal of Experimental Biology 2014 217: 453-462; doi: 10.1242/jeb.091801
  • RESEARCH ARTICLES
    Insulin-like peptides (AmILP1 and AmILP2) differentially affect female caste development in the honey bee (Apis mellifera L.)
    Ying Wang, Sergio V. Azevedo, Klaus Hartfelder, Gro V. Amdam
    Journal of Experimental Biology 2013 216: 4347-4357; doi: 10.1242/jeb.085779

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