Table 1.

Pre-exposure conditions for DSI in Series 2

ConditionsApical solutionsBasolateral solutions
Asymmetrical DSI
   ControlFWCM
   AmmFW, AmmCM, Amm
   CortFWCM, Cort
   Amm+CortFW, AmmCM, Amm+Cort
Symmetrical DSI
   ControlCMCM
   AmmCM, AmmCM, Amm
   CortCMCM, Cort
   Amm+CortCM, AmmCM, Amm+Cort
  • DSI, double seeded insert; FW, freshwater; CM, culture medium; Amm, 2000μ mol l–1 NH4Cl; Cort, 1000 ng ml–1 cortisol