Table 1.

Percentage survival (means±s.d.) of the tested tardigrade species after cooling at different rates from room temperature (RT) down to– 30°C with a constant thawing rate of 10°Ch–1 up to RT again

Species9°C h–17°C h–15°C h–13°C h–11°C h–1
M. sapiens 47.5±17.143.8±14.90.0±0.00.0±0.027.6±10.4
P. richtersi `group 1'90.0±10.877.5±10.432.5±11.9°16.3±14.927.5±5.0a
P. richtersi `group 2'23.8±8.57.5±5.05.0±4.815.0±10.838.8±11.9
P. richtersi `group 3'20.0±4.83.8±4.81.3±2.58.8±2.315.0±7.1
P. richtersi 92.5±9.666.3±8.555.0±14.7c61.3±19.372.5±6.5a,b
M. tonollii 35.0±8.20.0±0.027.5±13.235.0±8.276.3±12.5a
M. tardigradum 95.0±4.186.3±8.571.3±8.571.3±20.286.3±7.5
E. granulatus 53.1±3.246.9±7.522.5±11.452.5±8.460.6±4.3
E. testudo 46.9±5.538.8±6.319.4±5.517.5±5.442.5±4.6
  • Four replicates (N=4), containing 40 animals each, were used per cooling rate and species. aSignificant difference between 9 and 1°C h–1, bsignificant difference between 3 and 1°C h–1, and csignificant difference between 9 and 5°C h–1