Table 1.

PCR primers for cDNA cloning and semi-quantitative RT-PCR

Target genesApplicationSequences
Urea transporterDegenerate PCRSense: 5′-GTNCARAAYCCNTGGTGGRC-3′
Antisense: 5′-CCANGGRTTRTCRCANCCRTA-3′
5′-RACE PCRSense: 5′-ACATTCTAGAGGCCGAGGCG-3′
Antisense: 5′-CCACCAATAATAATCTCCTTTGTC-3′
3′-RACE PCRSense: 5′-TTCTTCCCTACGACGCTCATT-3′
Antisense:5′-ATTCTAGAGGCCGAGGCGGCCGACA-d(T)30N-3′
Semiquantitative RT-PCRSense: 5′-TTCTTCCCTACGACGCTCATT-3′
Antisense: 5′-TGCATTAGGAAGCCTAGTTAGAC-3′
GAPDH (internal standard)cRNA synthesisSense: 5′-ACAGATATGGAAGACAGTGAGTTTG-3′
Antisense: 5′-TGCATTAGGAAGCCTAGTTAGAC-3′
Semiquantitative RT-PCRSense: 5′-TCCACTCACGGTCGTTTTC-3′
Antisense: 59-TGACACCCATCACAAACATAGG-39