Table 1.

Composition of the Ringer's solutions (in mmol l–1) employed in the Ussing chamber studies

1234
SolutionNaCl/HCO3Cl freeHCO3 freeNa+ free
NaCl11001100
Sodium gluconate0110250
N-methyl-d-glucamine Cl000110
KCl5055
Potassium gluconate0500
MgSO40.50.50.50.5
CaCl21011
Calcium gluconate0100
NaHCO3252500
Choline bicarbonate00025
Hepes/Tris10101010
Pyruvate/glutamine4444
Gas95%O2: 5%CO295%O2: 5%CO2100%O295%O2: 5%CO2
pH7.47.47.47.4