Table 1.

Mass background isotope levels, injectate details, isotope dilution space, washout ratios, metabolic rate and water turnover rate for 6 green turtles used in DLW validation

TurtleL1L2L3L4R1R4Mean ± s.d.Sig. diff.
Mass (kg)
   Fed19.6025.0419.7625.8824.8419.3822.42±3.13a
   Fasted19.0425.1819.1025.3024.1019.2021.99±3.18a
   Post-fast 21 days19.4825.0619.4425.5024.3819.4622.22±3.04a
   Post-fast 23 days19.4225.2819.9226.2024.9219.8222.59±3.18a
   Post-fast 25 days19.9225.3820.1026.2025.2219.9022.79±3.10 a
Background 2H153.85154.59153.82153.57153.21153.12153.69±0.53
Background 18O1994.641995.181994.211994.671994.821994.511994.67±0.32
Initial injectate (moles)0.370.550.420.520.530.42
Injectate enrichment
   2H APE260611.09260611.09260611.09260611.09260611.09260611.09
   18O APE695197.53695197.53695197.53695197.53695197.53695197.53
Nd (ml)14047.4217593.3614821.4116709.7117019.6814014.7915701.06±1593.03
No (ml)13398.1616887.2414152.2215862.7916365.0013608.9115045.72±1508.41
   Dilution ratio Nd:No1.0481.0421.0471.0531.0401.0301.048±0.005
10 day fast 2H boost
   Injectate (moles)0.070.100.080.080.090.070.08±0.01
15 day fast 2H boost
   Injectate (moles)0.080.090.070.080.080.070.08±0.01
2H boost enrichment
   2H APE952572.52952572.52952572.52952572.52952572.52952572.52
Fed – kd6.399E–056.154E–058.009E–056.024E–055.836E–054.913E–056.223E–05±1.013E–05
Fed – ko7.297E–056.452E–058.653E–056.704E–056.298E–055.143E–056.758E–05±1.166E–05
Fasted – kd4.832E–053.799E–054.057E–053.925E–054.786E–054.218E–054.270E–05±4.406E–06
Fasted – ko4.462E–053.969E–053.997E–053.616E–053.500E–054.490E–054.006E–05±4.126E–06
   Fed – kd:ko0.880.95*0.930.900.930.96*0.91±0.02a
   Fasted – kd:ko1.080.961.021.091.370.941.07±0.16 b
Fed – respirometry (kJ kg–1 day–1)32.7024.14*33.8023.9824.1722.86*28.66±5.31a
Fed – DLW (kJ kg–1 day–1)59.56–8.76*19.2232.7811.85–8.06*30.85±21.01a
Fasted – respirometry (kJ kg–1 day–1)15.8914.4012.4314.1711.7314.0113.77±1.49b
Fasted – DLW (kJ kg–1 day–1)–65.80–6.60–34.19–44.01–130.351.63–46.55±47.90 n/a
Fed – RER0.880.820.780.930.740.850.83±0.07a
Fast (10 days) – RER0.580.600.240.460.500.780.53±0.18b
Fast (15 days) – RER0.690.690.580.580.480.520.59±0.09 b
Fed – breathing frequency0.140.090.10.090.10.070.10±0.02a
Fasted – breathing frequency0.110.060.090.080.060.120.09±0.03 a
% TBW (2H)
   Fed67.0667.0771.4861.1065.5167.7766.67±3.37a
   Fasted (10 days)59.4862.4966.3748.1850.5965.0758.70±7.63b
   Fasted (15 days)60.3160.6161.7253.5150.3161.5458.00±4.85 b
Water turnover (I day–1)
   Fed1.241.491.631.381.360.951.43±0.17a
   Fasted (10 days)0.790.860.750.690.830.760.78±0.06b
   Fasted (15 days)0.790.830.680.760.830.710.77±0.06b
   Fed (21 days)1.571.431.671.551.351.241.47±0.16a
   Fed (25 days)1.321.251.671.341.361.101.34±0.19 a
% TBW day–1
   Fed9.218.8611.538.688.407.089.57±1.33a
   Fasted (10 days)7.005.455.825.646.876.076.14±0.65b
   Fasted (15 days)6.945.485.835.686.866.076.14±0.62b
   Fed (21 days)13.749.4614.3411.5211.1410.6511.81±1.87c
   Fed (25 days)9.827.4711.798.418.358.259.02±1.56 a
  • Mass for fasted turtles is the average between days 10 and 15; post-fast 21 days is 21 days from start of trial and so on. The DLW MRs are from Eqn 7, see Materials and methods

    Mean ± s.d. and significant differences are given among related data groups (i.e. values within the lines of sig. diff. column). In the final column, letters (a,b,c) are used to denote significant differences. If the same letter is given then there is no difference, i.e. `a' is significantly different from `b' and `c' but not from another `a'. Values with an asterisk were not used in the determination of the mean