Table 2.

Water content in organs and carcasses of Rana sylvatica administered saline or saline containing urea, and sampled before or after freezing at –4°C

Saline treatedUrea loaded
Unfrozen
   Heart82.8±0.582.2±0.6
   Liver71.2±0.771.7±1.0
   Muscle79.2±0.278.5±0.4
   Carcass78.2±0.378.9±0.5
Frozen/thawed
   Heart83.9±0.383.4±0.7
   Liver78.8±0.4*78.3±0.5*
   Muscle79.2±0.478.3±0.5
   Carcass79.4±0.278.2±0.5
  • Water content is given as % fresh tissue mass. Values are means ± s.e.m.; N=5 frogs per group

    Asterisk denotes that the mean for the frozen/thawed group differed significantly (P<0.05) from its unfrozen counterpart