Table 2.

Alexa Fluor dyes used to label antibodies against Na+/K+-ATPase, NKCC1a, NCC, NHE3 and CFTR

Alexa FluorEx/Em* (nm)Ex/Em (nm)Pseudocolor
Na+/K+-ATPase488495/519488/505-530Red
NKCC1a430434/539405/>530Blue
NCC405402/421405/420-480Cyan
NHE3555555/565543/560-615Yellow
CFTR647650/668633/>650Green
  • * Wavelength of maximum excitation and emission of each Alexa Fluor dye

  • Wavelength of laser excitation and recorded emission for confocal laser-scanning microscopy