Table 1.

Antibodies used for immunohistochemistry

AntibodyHostWorking dilutionAntigen (peptide sequence in brackets)Source
PrimaryeNOS N30030Rabbit1:500Human eNOS c-terminal (1030-1209)1
iNOS N32030Rabbit1:1000Mouse iNOS c-terminal (961-1144)1
nNOS N31030Rabbit1:100Human nNOS c-terminal (1095-1289)1
nNOS SC1025Rabbit1:150Human/mouse nNOS n-terminal2
uNOS PA1-039Rabbit1:100Murine iNOS and nNOS c-terminal (1113-1122)3
nNOS B220Rabbit1:400Rat nNOS c-terminal4
Ser5545Rabbit1:4000Serotonin5
SecondaryDaR-biotin 711-065-152Donkey1:4006
DaR-FITC 711-095-152Donkey1:1006
  • All the NOS antibodies are polyclonal. eNOS, endothelial NOS; iNOS, inducible NOS; nNOS, neuronal NOS; uNOS, universal NOS – i.e. antibody reacting to both iNOS and nNOS. Sources: (1) BD Biosciences (Transduction Laboratories), San Jose, CA, USA; (2) Santa Cruz Biotechnology, Santa Cruz, CA, USA; (3) Affinity BioReagents, Golden, CO, USA; (4) Euro-Diagnostica, Malmö, Sweden; (5) Sigma Chemical Company, St Louis, MO, USA; (6) Jackson ImmunoResearch Laboratories, West Grove, PA, USA