Table 2.

Accumulation of GABA and the percent of GABA accumulation that can be accounted for by reduction in the amount of four amino acids

d.p.f.GlutamateGlutamineAspartateAsparagineTotalGABA%
4–1.8±0.40–0.6±0.10–2.45.3±3.045.3
8–10.6±0.20–0.3±0.050–10.922.9±7.347.6
16–14.9±3.6–2.4±0.7–0.5±0.2–0.4±0.09–8.221.5±3.484.2
32–4.0±0.5–5.9±0.5–0.6±0.1–0.3±0.08–10.815.0±3.172.5
  • Values are in nmol embryo-1 (mean ± s.e.m., N=3). Values were calculated by subtracting the amount of each amino acid at time=0 (normoxia) from the amount present at terminal sampling, which was 21 days of anoxia for 4 d.p.f. embryos and 60 days of anoxia for 8, 16 and 32 d.p.f. embryos