Table 1.

Primers and probes used in digoxigenin (DIG) PCR probe labelling and real-time RT–PCR (TaqMan assays)

MethodPrimerOligonucleotidesConcentration (nmol l-1)Amplicon (bp)
DIG PCR probe labellingLuc 1Forward5′-caaatcattccggatactgcg-3′400397
Reverse5′-cccggtttatcatccccct-3′400
Real-time PCR assaysLuc 2Forward5′-tgggctcactgagactacatca-3′90064
Reverse5′-cgcgcccggtttatcatc-3′900
ProbeFAM-tcgggtgtaatcagaatag250
Real-time PCR assays18SForward5′-gccctgtaattggaatgagtgtact-3′900101
Reverse5′-acgctattggagctggaattacc-3′900
ProbeFAM-cttgccctccaattgg250