Table 1.

The fractionator sampling parameters, mean values

BA (μm)1/ssf∑secta(frame) (μm2)Step size (x,y) (μm)1/asf∑dishdis (μm)tQ- (μm)1/hsfEmbedded ImageEmbedded Image
Göttingen minipig
    Neonate405011-13749160034171721539.12.6253383
    Adult4010011-122497260027071682037.71.9289337
Domestic pig
    Neonate401008-9999180032421411538.42.6260-
    Adult402007-82706280028961372537.91.5235-
  • Abbreviations: BA, block advance; ssf, section sampling fraction;∑ sect, number of sections; a(frame), area of counting frame;∑ dis, number of disectors hitting neocortex; hdis, disector height; tQ-, q-weighted section thickness; hsf, height sampling fraction; Embedded Image, number of neurons counted; Embedded Image, number of glial cells counted.

    The a(frame) for glial cell estimations in adult Göttingen minipigs was 50% of the original frame.