Table 3.

Gene expression of ion transporters that did not change after freshwater transfer, measured by qRT-PCR

Time after transfer
GeneSalinity12 h3 days7 days
Gills
    Na+,K+-ATPaseα 1aBW1.0±0.11.1±0.11.1±0.1
FW0.9±0.11.0±0.31.5±0.2
    NBC1BW1.0±0.11.0±0.10.8±0.1
FW1.0±0.11.0±0.10.7±0.1
    V-ATPaseBW1.0±0.11.1±0.11.4±0.1
FW1.0±0.11.1±0.11.2±0.2
Opercular epithelium
    Na+,K+-ATPaseα 1aBW1.0±0.21.3±0.21.0±0.3
FW1.1±0.22.2±0.61.3±0.3
    NBC1BW1.0±0.10.5±0.1†0.6±0.1†
FW0.9±0.10.5±0.1†0.6±0.1†
    V-ATPaseBW1.0±0.41.3±0.20.9±0.2
FW1.0±0.20.9±0.20.7±0.1
  • Values are means ± s.e.m. N≥7.† Significant difference from 12 h brackish water control (P<0.05). qRT-PCR expression data are normalized to 12 h brackish water controls.

    BW, brackish water; FW, freshwater.