Table 1.

Primers used for cloning

GeneDirectionSequence (5′-3′)Tm (°C)SpeciesAccession no.
CA2F(C/T)CG (C/G)CA GTC (C/T)CC (C/T/A)(A/G)T (T/A/G)GA50 Danio rerio NM_199215.1
RCAG (A/T)GG (A/T/G/C)GG (T/A/G)GT GGT C(A/C)G (G/A)G50 Mus musculus NM_009801.3
Oncorhynchus mykiss AY5414870
Platichthys flesus AF093622
Tribolodon hakonensis AB055617
NBC1FGC(G/C) AG(G/A) TGG AT(A/T/C) AAG TTT GA(G/A) GA52 Ambystoma tigrinum AF001958
RGA(G/A/C) CG(C/A) AGG TTG GA(C/T) TT(C/T) TTG53 Mus musculus AF141934
Oncorhynchus mykiss AF434166
Tribolodon hakonensis AB055467
NHE2FAA(C/T) GA(C/T) G(C/G)I GTI ACI GTI GTSee Towle et al. (1997)
RGG IC(G/T) IAT IGT IAT ICC (C/T)TG
NHE3FGC(C/G/A/T) GT(G/T) CTG GC(C/G/A/T) GTI TT(C/T) GA(G/A) GASee Hirata et al. (2003)
RGC(C/G/A/T) CC(C/G/A/T) C(G/T)(C/G/A/T) A(G/A)I CCI CC(G/A) TA
  • F, forward; R, reverse; Tm, annealing temperature.