Comparison of the catalytic and kinetic properties of cytoplasmic carbonic anhydrase isozymes from rainbow trout, using gill tissue and red blood cells as enzyme sources
| Km (mmol l−1) | kcat (e4 s−1) | Ki Az (nmol l−1) | Ki Cl− (mmol l−1) | |
|---|---|---|---|---|
| Gill | 8.72±1.03 | 8.90±1.95 | 1.21±0.18 | 62.03±6.75 |
| RBC | 23.26±5.22* | 30.28±5.83* | 1.34±0.10 | 61.34±2.41 |
Values are mean±s.e.m. (N=4), with statistically different groups indicated by an asterisk (unpaired t-test; P<0.05). RBC, red blood cells.