Table 5.

Percent systemic cardiac output (%Q̇sys) and absolute blood flow (μl min−1 kg−1) to the various tissues of 5°C- and 21°C-acclimated turtles during routine normoxic and anoxic conditions and following anoxic injections of phenylephrine and phentolamine

%sys Tissue flow (μl min−1 g−1)
Acclimation temperatureTissueRelative mass (%)Routine normoxiaRoutine anoxiaPhenylephrine (50 μg kg−1)Phentolamine (3 mg kg−1)Routine normoxiaRoutine anoxiaPhenylephrine (50 μg kg−1)Phentolamine (3 mg kg−1)
5°CIntegument4.4±0.26.3±1.36.1±1.13.4±0.45.3±0.820.9±4.9a5.0±1.02.6±0.56.6±1.9
Muscle19.7±0.911.1±1.512.3±1.4a17.0±2.9b19.1±1.8b8.7±1.7a2.4±0.42.9±0.65.5±1.4
Bone6.6±0.68.2±0.97.2±1.05.5±1.19.1±0.723.0±5.1a4.7±1.03.3±0.88.8±2.0
Stomach2.3±0.42.6±1.31.2±0.30.8±0.21.0±0.431.8±18.1a2.4±0.51.6±0.42.2±0.7
Intestines4.8±0.84.2±0.83.6±1.53.0±1.04.1±1.617.8±4.8a3.3±1.32.5±0.84.9±1.7
Spleen0.3±0.11.6±0.90.5±0.10.6±0.40.5±0.2100.0±50.78a9.8±3.28.0±3.913.9±5.9
Ventricle0.2±0.020.7±0.30.8±0.30.6±0.20.8±0.438.4±15.5a11.1±4.18.2±3.213.3±5.9
Atria0.07±0.020.2±0.050.4±0.10.5±0.080.5±0.140.1±9.1a17.4±4.219.7±5.222.8±4.2
Brain0.3±0.20.7±0.30.9±0.40.3±0.090.3±0.2121.4±43.3a38.1±16.813.2±4.615.2±11.3
Liver9.4±0.64.8±1.18.3±2.1*a27.3±6.7b9.1±1.4a9.3±2.2a3.7±0.9b12.8±5.5a5.8±1.3a,b
Kidneys0.9±0.36.0±3.22.7±1.7*b3.2±2.11.0±0.2223.7±160.1a15.8±8.416.8±9.99.3±3.5
Gonads8.7±2.66.9±2.52.6±0.4*3.4±1.63.2±0.716.5±4.9a3.2±1.62.5±1.26.3±3.5
Fat2.9±0.61.8±0.41.9±0.71.9±0.82.3±0.77.3±1.5a1.8±0.42.0±0.93.5±1.3
Connective tissue1.9±0.21.7±0.40.7±0.20.8±0.21.3±0.321.9±4.1a2.6±0.72.5±0.55.9±1.5
Shell35.2±2.141.8±7.550.2±4.9*a31.3±5.5b42.5±3.0c19.6±6.5a5.2±0.93.0±0.96.4±1.7
Eyes0.07±0.021.4±0.50.6±0.30.2±0.10.1±0.02347.8±118.0a41.4±17.813.5±6.412.6±3.0
21°CIntegument4.5±0.12.5±0.44.5±1.05.4±0.84.3±0.632.1±7.220.4±7.120.4±3.726.6±5.5
Muscle20.2±0.516.0±3.820.9±7.0*16.6±3.322.5±6.041.0±11.6a21.7±8.7b16.0±4.9b33.5±12.3a,b
Bone6.0±0.27.2±1.010.7±1.68.0±1.812.9±4.858.9±11.3a28.8±5.3a,b19.3±3.1b45.4±12.1a,b
Stomach1.7±0.26.2±4.21.0±0.3*3.3±1.40.8±0.384.9±31.3a7.2±1.8b21.0±6.3b9.4±3.4b
Intestines4.0±0.415.6±3.54.1±1.4*10.4±7.43.5±1.1185.2±59.9a12.9±2.931.4±19.317.0±2.5
Spleen0.2±0.020.7±0.30.2±0.10.6±0.30.3±0.1217.5±116.58.5±4.041.3±30.724.0±11.3
Ventricle0.2±0.018.8±5.46.1±3.11.6±0.73.9±2.62354.4±1653.6326.2±125.089.7±27.9294.3±151.1
Atria0.1±0.071.0±0.60.8±0.50.3±0.090.4±0.12250.5±1798.2491.4±438.4110.4±48.7270.9±197.5
Brain0.08±0.010.4±0.23.8±1.90.6±0.70.5±0.5145.3±71.5480.1±305.595.8±81.23.6±21.2
Liver8.4±1.04.3±0.82.7±0.99.5±5.14.9±1.823.7±4.35.3±2.122.0±15.414.9±6.8
Kidneys0.6±0.054.3±2.51.2±0.50.4±0.20.8±0.2317.9±176.7a29.8±14.310.1±4.728.7±9.6
Gonads8.5±2.35.0±1.91.9±0.84.6±1.92.8±0.939.5±9.642.6±39.348.5±38.859.6±5.14
Fat3.7±0.81.1±0.31.2±0.31.9±0.71.9±0.418.4±4.38.3±2.811.1±4.017.9±4.0
Connective tissue1.2±0.23.0±1.90.7±0.11.0±0.11.4±0.277.9±48.37.2±1.910.7±3.123.3±7.0
Shell38.0±1.223.7±6.739.2±5.2*35.9±7.939.2±7.335.5±11.419.9±4.217.5±4.029.7±6.5
Eyes0.06±0.010.3±0.11.1±0.60.1±0.060.06±0.04497.0±236.81450.5±1296.280.4±52.139.1±2.14
  • Values are means ± s.e.m. (N=6 at 5°C; N=5 at 21°C).

    Significant differences (P<0.05) in % sys for each tissue between routine anoxia, phenylephrine and phentolamine injections are indicated with dissimilar letters.

    Significant differences (P<0.05) in absolute blood flow for each tissue between the four conditions are indicated by dissimilar letters.

  • * Significant differences (P<0.05) in % sys for each tissue between routine normoxia and routine anoxia.

  • Significant differences (P<0.05) in normoxic % sys and absolute blood flow between acclimation temperature.