Table 1.

Oligonucleotide primers employed to amplify carbonic anhydrase fragment used for in situ hybridization with riboprobe

Primer setNucleotide sequence (5′→3′)Position in cDNA of A. elegantissima
Degenerate sense primer CA4AGAG CAR TTY CAY TTY CAY TGG282-303
Degenerate antisense primer CA8CGG ART ANG TCC ART ART C582-601
  • Anthopleura elegantissima carbonic anhydrase cDNA GenBank Accession number: AF140537.

    N, A/C/G/T; R, A/G; Y, C/T.