Toxicity of recombinant and Bacillus thuringiensis subsp. israelensis (Bti) 4Q7 strains producing the B. sphaericus (Bs) binary toxin, B. thuringiensis Cry 11B and/or Cyt1A against fourth instar Culex quinquefasciatus and Aedes aegypti in comparsion with wild-type strains
Strain | Toxin combination | LC50 (fiducial limits) (ng ml−1) | Slope (± s.e.m.) |
---|---|---|---|
Culex quinquefasciatus | |||
4Q7/pPFT11Bs-CRP | Cry11B | 9.2 (6.8-12.1) | 2.0±0.2 |
4Q7/p45S1 | Bin + Cyt1A | 3.7 (2.5-5.2) | 1.5±0.2 |
4Q7/p45S1-11B | Cry11B + Bin + Cyt1A | 1.7 (1.2-2.5) | 1.5±0.2 |
Bti IPS-82 | Cry4A + Cry4B + Cry11A + Cyt1A | 7.9 (6.2-9.7) | 3.4±0.6 |
Bs 2362 | Bin | 12.6 (8.7-17.5) | 1.4±0.2 |
Aedes aegypti | |||
4Q7/pPFT11Bs-CRP | Cry11B | 28.5 (17.7-45.2) | 2.6±0.5 |
4Q7/p45S1 | Bin + Cyt1A | 265.5 (214.9-321.6) | 3.0±0.4 |
4Q7/p45S1-11B | Cry11B + Bin + Cyt1A | 37.2 (30.5-44.8) | 3.0±0.4 |
Bti IPS-82 | Cry4A + Cry4B + Cry11A + Cyt1A | 10.2 (8.0-21.9) | 5.6±1.6 |
Bs 2362 | Bin | Not tested | - |