Table 3.

Activity of citrate synthase, carnitine palmitoyl transferase and cytochrome c oxidase in mitochondrial suspensions, expressed in U mg−1 mitochondrial protein

Citrate synthase Carnitine palmitoyl transferase Cytochrome c oxidase
Step5°C15°C5°C15°C5°C15°C
Warm acclimation
Initial0.74±0.05c1.02±0.03c0.014±0.002a,b0.019±0.001c1.37±0.09a1.73±0.09
Week 11.02±0.09a1.4±0.1a0.015±0.001a,b0.032±0.003a,b1.4±0.1a,b1.8±0.1
Week 20.98±0.04a,b1.54±0.05a0.0192±0.0009a0.036±0.001a1.8±0.1a2.1±0.1
Week 30.77±0.04b,c1.31±0.06a,b0.011±0.001b0.027±0.002b1.31±0.09b1.8±0.1
Week 40.64±0.04c1.05±0.06b,c0.012±0.002b0.026±0.003a,b,c1.4±0.1a,b1.6±0.2
Week 80.62±0.03c1.04±0.05c0.0121±0.0009b0.025±0.002b,c1.5±0.1a,b1.75±0.09
Cold acclimation
Initial0.59±0.02b1.01±0.060.0094±0.0005b0.020±0.001b1.35±0.08d1.58±0.09d
Week 10.59±0.03b1.01±0.050.0106±0.0005b0.022±0.001b1.6±0.2c,d1.8±0.2c,d
Week 20.69±0.03a1.17±0.060.0139±0.0006a0.028±0.001a1.93±0.05b,c2.21±0.07b,c
Week 60.73±0.03a1.20±0.040.0160±0.0005a0.027±0.001a2.40±0.09a2.8±0.1a
Week 100.66±0.02a,b1.09±0.040.0153±0.0006a0.028±0.001a2.19±0.07a,b2.56±0.10a,b
  • Data are means ± s.e.m. and are compared between acclimation steps within each experiment for each assay temperature. When values in a column are followed by different letters, they are significantly different (P≤0.05; ANOVA and a Tukey's a posteriori test).