Table 4.

Compounds identified in the trichloroacetic acid (TCA)-soluble organic fraction of the anemone tissue from A. viridis after double compound incubation

Intensity of radioactivity *
FractionCompoundNaH14CO3Succinate + glucoseFumarate + glucoseSuccinate + glycerolFumarate + glycerol
NeutralFructose-++--
Glucose++++++++++
Sucrose-++++
Glycerol---++++++
AcidicGlucose 6-phosphate+++++++++++++++
Unknown 1 ++++++
Unknown 2 +++++++++
Citrate+++++++
Fumarate+-+--
Malate++++++++++++
Succinate+++++-++++
BasicAlanine---++
Aspartate+++++++++
Glutamate+++++
Glutamine+++++
Lysine+++++--
Proline+++++--
Threonine++++++++
Unknown 3 ---++++++
  • * Experiments were performed in the presence of 5 μmol l-1 dichlorophenyl dimethyl urea (DCMU). Compounds identified after incubation in NaH14CO3 (data from Table 1) included for comparison. Symbols as in Table 1

  • Unknowns 1 and 2 as in Table 1. Unknown 3 co-eluted with other amino acids but no positive identification could be made