Table 1.

Compounds identified in the trichloroacetic acid (TCA)-soluble organic fractions of separated algal cells and animal tissue from A. viridis incubated under photosynthesizing conditions with NaH14CO3 for 5 min

Intensity of radioactivity *
FractionCompoundAlgal cellsAnimal tissue
NeutralFructose++-
Glucose+++++
Sucrose++-
Glycerol+-
AnionicGlucose 6-phosphate++++++
Unknown 1 +++++
Unknown 2 ++++++
Citrate++++
Fumarate++
Malate++++
Succinate+++
BasicArginine+-
Aspartate++
Asparagine+++-
Glutamate++++
Glutamine+++
Isoleucine+-
Lysine++
Serine+-
Proline-+
Threonine+++
Tyrosine+-
Valine++-
  • * Radioactivity in individual compounds was detected using autoradiography with spots identified by comparison with authentic compounds. +++, intense signal; ++, strong signal; +, detectable; -, not detected (these symbols apply relative to other compounds within each fraction and should not be compared between fractions; intensity should also not be compared between columns - algal samples had much more intense labelling overall)

  • Unknowns 1 and 2 were compounds that co-eluted with other sugar phosphates but could not be positively identified