Table 1.

Effects of anisosmotic conditions, ion substitution and transport inhibitors on intracellular pH (pHi) of trout hepatocytes

TreatmentBaselineBeforeΔpHiAfter 15 minΔpHi
Hyposmotic (n=54)7.39±0.037.20±0.03-0.19±0.02
    Na+-free (n=20)7.43±0.077.33±0.08*-0.10±0.037.25±0.10-0.08±0.04
    EIPA (n=35)7.44±0.027.21±0.03*-0.22±0.037.01±0.03-0.20±0.03
    Cl--free (n=31)7.48±0.077.86±0.08*0.37±0.057.93±0.080.08±0.05
    SITS (n=75)7.48±0.027.21±0.02*-0.27±0.037.12±0.02-0.10±0.02
Hyperosmotic (n=67)7.50±0.047.93±0.040.42±0.02
    Na+-free (n=39)7.23±0.046.98±0.06*-0.24±0.036.96±0.09-0.02±0.04
    EIPA (n=33)7.30±0.046.91±0.02*-0.39±0.046.91±0.030.00±0.03
    Cl--free (n=27)7.47±0.047.88±0.06*0.41±0.038.13±0.060.25±0.04
    SITS (n=77)7.43±0.027.34±0.03*-0.10±0.027.51±0.030.18±0.02
  • Baseline, before and after 15 min denote initial pHi, pHi before anisosmotic exposure and pHi after 15 min of anisosmotic exposure, respectively. ΔpHi indicates change of pHi induced by exposure to transport inhibitors, ion-substituted or anisosmotic medium. Data are means± S.E.M. of n cells. Ion transport inhibitors EIPA and SITS were applied at concentrations of 100 μmol l-1 and 500 μmol l-1, respectively.

  • * P<0.05, significantly different from baseline pHi; P<0.05, significantly different from pHi before anisosmotic exposure; P<0.05, significantly different from ΔpHi in controls.