RT Journal Article
SR Electronic
T1 Identification of novel circadian transcripts in the zebrafish retina
JF The Journal of Experimental Biology
JO J. Exp. Biol.
FD The Company of Biologists Ltd
SP jeb192195
DO 10.1242/jeb.192195
VO 222
IS 1
A1 Ramasamy, Soundhar
A1 Sharma, Surbhi
A1 Iyengar, Bharat Ravi
A1 Vellarikkal, Shamsudheen Karuthedath
A1 Sivasubbu, Sridhar
A1 Maiti, Souvik
A1 Pillai, Beena
YR 2019
UL http://jeb.biologists.org/content/222/1/jeb192195.abstract
AB High fecundity, transparent embryos for monitoring the rapid development of organs and the availability of a well-annotated genome has made zebrafish a model organism of choice for developmental biology and neurobiology. This vertebrate model, which is also a favourite in chronobiology studies, shows striking circadian rhythmicity in behaviour. Here, we identify novel genes in the zebrafish genome that are expressed in the zebrafish retina. We further resolve the expression pattern over time and tentatively assign specific novel transcripts to retinal bipolar cells of the inner nuclear layer. Using chemical ablation and free run experiments, we segregate the transcripts that are rhythmic when entrained by light from those that show sustained oscillations in the absence of external cues. The transcripts reported here with rigorous annotation and specific functions in circadian biology provide the groundwork for functional characterization of novel players in the zebrafish retinal clock.