ABSTRACT
Genetically engineered mouse models have been used to determine the role of sarcolipin (SLN) in muscle. However, a few studies had difficulty in detecting SLN in FBV/N mice and questioned its relevance to muscle metabolism. It is known that genetic alteration of proteins in different inbred mice strains produces dissimilar functional outcomes. Therefore, here we compared the expression of SLN and key proteins involved in Ca2+ handling and mitochondrial metabolism between FVB/N and C57BL/6J mouse strains. Data suggest that SLN expression is less abundant in the skeletal muscles of FVB/N mice than in the C57BL/6J strain. The expression of Ca2+ transporters in the mitochondrial membranes was also lower in FVB/N than in C57BL/6J mice. Similarly, electron transport chain proteins in the mitochondria were less abundant in FVB/N mice, which may contribute to differences in energy metabolism. Future studies using different mouse strains should take these differences into account when interpreting their data.
Footnotes
Competing interests
The authors declare no competing or financial interests.
Author contributions
Conceptualization: S.S., M.P., N.C.B.; Methodology: S.S., N.C.B.; Software: S.S.; Validation: S.S.; Formal analysis: S.S., N.C.B.; Investigation: S.S., M.P., N.C.B.; Resources: N.C.B.; Data curation: N.C.B.; Writing - original draft: S.S., M.P., N.C.B.; Writing - review & editing: S.S., M.P., N.C.B.; Supervision: M.P., N.C.B.; Project administration: M.P., N.C.B.; Funding acquisition: M.P.
Funding
N.C.B. is funded by the Science and Engineering Research Board, Department of Science and Technology, India (grant no. ECR/2016/001247) and Department of Biotechnology, Ministry of Science and Technology, India (grant nos BT/RLF/Re-entry/41/2014 and BT/PR28935/MED/30/2035/2018). M.P. is supported in part by the National Institutes of Health (grants nos R01-HL 088555 and R01 DK098240-01). Deposited in PMC for release after 12 months.
- Received October 4, 2020.
- Accepted November 23, 2020.
- © 2021. Published by The Company of Biologists Ltd
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