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Research Article
Hypoxia-induced changes in hemoglobins of Lake Victoria cichlids
Guido van den Thillart, Inger Wilms, Maaike Nieveen, Roy E. Weber, Frans Witte
Journal of Experimental Biology 2018 221: jeb177832 doi: 10.1242/jeb.177832 Published 10 September 2018
Guido van den Thillart
1Institute of Biology Leiden, Department of Molecular Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands
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  • ORCID record for Guido van den Thillart
  • For correspondence: guidovandenthillart@gmail.com
Inger Wilms
1Institute of Biology Leiden, Department of Molecular Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands
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Maaike Nieveen
1Institute of Biology Leiden, Department of Molecular Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands
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Roy E. Weber
2Zoophysiology, Department of Biological Sciences, Aarhus University, C. F. Møllers Allé 1131, DK 8000 Aarhus, Denmark
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Frans Witte
1Institute of Biology Leiden, Department of Molecular Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands
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    Fig. 1.

    Thin-layer isoelectric focusing (IEF)-gel analysis. A typical IEF gel showing isoelectric point (pI) calibration proteins (lanes 1 and 8), and the isohemoglobins (isoHbs) of Haplochromis thereuterion siblings raised for approximately 10 months under normoxia (NR, 100% AS; lanes 4 and 7) or hypoxia (HR, 10% AS; lanes 2 and 6), and specimens that were subjected to reversed acclimation (HR>NR and NR>HR) for an additional month (lanes 3 and 5). The gels were scanned and the numbers of the ten isoHb bands with the highest intensities are shown.

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    Fig. 2.

    Average relative intensity of the ten largest isoHb bands of four cichlid species. (A) H. thereuterion, (B) Haplochromis ishmaeli, (C) Platytaeniodus degeni and (D) Haplochromis piceatus. Asterisk indicates a significant difference (*P<0.05) between normoxia-raised (NR; white bars) and hypoxia-raised (HR; black bars) siblings. See Table 1 for sample numbers and condition. The x-axis gives the isoHb band numbers. Values are means±s.d.

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    Fig. 3.

    Oxygen equilibrium curves. Shown are the O2 equilibrium curves of stripped hemoglobin (Hb) of HR (black symbols) and NR (white symbols) H. thereuterion in the absence (squares) and presence of ATP (pyramidal triangles) and GTP (inverted triangles) at pH 6.97 (A) and pH 7.62 (B). Hb samples were prepared from pooled blood samples from at least five animals. Temperature, 25°C; tetrameric Hb concentration, 0.14; ATP/Hb4 and GTP/Hb4 ratio, 19.2.

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Keywords

  • Isohemoglobins
  • Hemoglobin affinity
  • Chronic hypoxia
  • Acclimation
  • Species flock
  • Cichlids

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Research Article
Hypoxia-induced changes in hemoglobins of Lake Victoria cichlids
Guido van den Thillart, Inger Wilms, Maaike Nieveen, Roy E. Weber, Frans Witte
Journal of Experimental Biology 2018 221: jeb177832 doi: 10.1242/jeb.177832 Published 10 September 2018
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Research Article
Hypoxia-induced changes in hemoglobins of Lake Victoria cichlids
Guido van den Thillart, Inger Wilms, Maaike Nieveen, Roy E. Weber, Frans Witte
Journal of Experimental Biology 2018 221: jeb177832 doi: 10.1242/jeb.177832 Published 10 September 2018

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