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- Fig. 1.
Thin-layer isoelectric focusing (IEF)-gel analysis. A typical IEF gel showing isoelectric point (pI) calibration proteins (lanes 1 and 8), and the isohemoglobins (isoHbs) of Haplochromis thereuterion siblings raised for approximately 10 months under normoxia (NR, 100% AS; lanes 4 and 7) or hypoxia (HR, 10% AS; lanes 2 and 6), and specimens that were subjected to reversed acclimation (HR>NR and NR>HR) for an additional month (lanes 3 and 5). The gels were scanned and the numbers of the ten isoHb bands with the highest intensities are shown.
- Fig. 2.
Average relative intensity of the ten largest isoHb bands of four cichlid species. (A) H. thereuterion, (B) Haplochromis ishmaeli, (C) Platytaeniodus degeni and (D) Haplochromis piceatus. Asterisk indicates a significant difference (*P<0.05) between normoxia-raised (NR; white bars) and hypoxia-raised (HR; black bars) siblings. See Table 1 for sample numbers and condition. The x-axis gives the isoHb band numbers. Values are means±s.d.
- Fig. 3.
Oxygen equilibrium curves. Shown are the O2 equilibrium curves of stripped hemoglobin (Hb) of HR (black symbols) and NR (white symbols) H. thereuterion in the absence (squares) and presence of ATP (pyramidal triangles) and GTP (inverted triangles) at pH 6.97 (A) and pH 7.62 (B). Hb samples were prepared from pooled blood samples from at least five animals. Temperature, 25°C; tetrameric Hb concentration, 0.14; ATP/Hb4 and GTP/Hb4 ratio, 19.2.
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