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Figures
- Fig. 1.
Fluorescent microspheres dispersing through the caudal region of the glass catfish secondary vascular system (SVS). Microspheres that were 0.02 μm (green; A) and 4 μm (green; B) were small enough to pass into the SVS. A white box in A is enlarged to represent the body segment illustrated in B. CH, caudal heart.
- Fig. 2.
Light microscope images of the ventral-most vessel of the glass catfish secondary vascular system (SVS). In all images, the top of the panel represents the dorsal side of the fish, bottom is ventral, right is posterior and left is anterior. The arrow in each panel indicates the ventral-most vessel of the SVS and typical direction of flow. Each stage (0–3) of red blood cell (RBC) dispersal into the SVS is depicted in the separate panels. Stage 0: there are no RBCs in the SVS; Stage 1: few RBCs in the SVS, little or no flow; Stage 2: RBCs starting to enter the SVS, individual cells can still be identified, flow is noticeable but slow; Stage 3: rapid flow of densely packed RBCs through the SVS.
- Fig. 3.
Changes in haematocrit and condition factor in glass catfish upon and following exposure to hypoxia [no aquatic surface respiration (ASR)] and exercise. Data are plotted as means ± s.e.m. Haematocrit was measured at rest, immediately following treatment and following 1 h recovery. A different group of fish was used for each treatment, i.e. not repeated measures. The number of replicates (N) is noted for each sampling time, and different letters denote significant differences within treatment (ANOVA). Inset shows no change in the fish condition factor [K=100(W/L3)] at each sampling.
- Fig. 4.
Changes in secondary vascular system (SVS) flow patterns by stage (0–3). See Fig. 2 for stage descriptions. The stage of the SVS was observed immediately (Imm.) or 1 h following exposure (1 h). The y-axis represents the percentage of fish observed exhibiting each stage of the SVS within a treatment group (x-axis). The number of replicates (N) is noted at the top of each bar for each treatment. ASR, aquatic surface respiration; Iso, isoproterenol; Prop, propranolol; PHT, phentolamine.
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