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Figures
- Fig. 1.
(A) Heat map showing the normalized expression (log2-ratio) of the 1531 genes that had significant temperature effects (ANOVA, P<0.01). Genes are ordered in rows, based on their Spearman correlation to the expression of the highest expressed gene (top most). The color scale bar indicates log2-ratio, with blue down-regulation, yellow up-regulation, and black no change compared with the median of the control samples for each respective temperature-species group. Sample size, N=6 for each species at each sampling temperature. (B) Principal component loading plot of principal component 1 (PC1) versus principal component 5 (PC5).
- Fig. 2.
Annotated heat maps showing the expression of genes that belong to significant Gene Ontology (GO) biological processes from the gene group correlation analysis (GCA). (A) Genes in the protein folding biological process. Genes are ordered based on the Spearman correlation of their expression to that of the highest expressed gene in this category (DnaJ B4). (B) Genes in the proteolysis biological process. Genes are ordered based on the Spearman correlation of their expression to that of caspase-3.
- Fig. 3.
Average normalized expression (log2-ratio) among seven Hsp70 genes (Hsp70s) in response to acute heat stress. Error bars indicate the standard error.
- Fig. 4.
Hierarchical clustering and annotated heat map showing clustering and normalized expression (log2-ratio) of 96 genes that had significant species × temperature interactions. The dendrogram on the left shows the clustering of genes based on expression pattern, and the four main clusters are numbered and marked by horizontal gray bars. The color scale bar indicates log2-ratio, with blue down-regulation, yellow up-regulation, and black no change in expression compared with the median of the control samples for each respective temperature–species group. Sample size, N=6 for each species at each sampling temperature. References for hypothesized gene functions are listed in supplementary material Table S1.
- Fig. 5.
Average normalized expression (log2-ratio) among eight genes encoding subunits of the proteasome in response to acute heat stress. Error bars indicate the standard error.
- Fig. 6.
Scatter plot of the expression values of all molecular chaperone genes that were significantly differentially expressed, including seven genes of Hsp70, two genes of Hsp90, Hsp60, two genes of DnaJ, eight genes of chaperonin TCP1 (all labeled as gray circles), and Hsp24 (colored triangles). Each data point represents the average normalized log2-ratio in both M. trossulus and M. galloprovincialis of one gene at one temperature (13°C, 24°C, 28°C or 32°C). Vertical error bars indicate the standard error among M. galloprovincialis samples and horizontal error bars indicate the standard error among M. trossulus samples.
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