Article Figures & Tables
Figures
- Fig. 1.
Attraction of A. variegatum to rumen fluid odour. Polar plots of the mean walking direction of 18 A. variegatum adults presented in three successive 1 min intervals with (A) a control air stream (0°), (B) rumen fluid odour in the air stream and (C) the control air stream (without rumen fluid) again. Ticks that spent more time walking in the cone delimited by 60° either side of upwind (in red) after presentation of the rumen odour were considered to be attracted. (D) A track described by an A. variegatum adult in the same experiment; the track started (filled circle) with the tick walking down or across wind in the control period; the arrows on the track indicate stimulus on and off; the bar represents a displacement of 20 cm. Note the local search behaviour induced in A. variegatum by withdrawal of the rumen odour from the air stream. Open arrow at bottom indicates airstream direction (0°) for A,B,C and D.
- Table 2.
Responses of adult A. variegatum to entrained rumen odour and to individual chemostimulants of the rumen fluid odour and their mixtures
Treatment Ticks tested (N) Source dose % Attraction1 % Change in speed1 Entrained rumen fluid odour 17 1/10 dilution +42***,a +4e,f Single components Butanoic acid 16 10 ng +34***,a,b –34**,f,g Isobutanoic acid 29 10 ng–1 μg +32***,a,b –9*,e,f 4-Methylphenol 11 10 ng +24**,b,c –2e 3-Methylindole 20 10 ng +12*,b,c –7e,f Acetic acid 14 1 μg +15*,b,c +3e Propanoic acid 20 10 ng +22***,a,b –12***,f Mixtures Butanoic acid:isobutanoic acid:4-methylphenol: 3-methylindole 18 1 μg:100 ng:10 ng:10 ng +25***,a,b –3e,f Butanoic acid:isobutanoic acid:4-methylphenol: 3-methylindole 11 1 μg:10 ng:10 ng:10 ng +3c –5e,f Butanoic acid:isobutanoic acid:4-methylphenol: 3-methylindole 20 10 ng:10 ng:10 ng:10 ng +2c +6e 2Methane 13 70 ppm +1c –6e,f - Fig. 2.
Identification of rumen volatiles that stimulate olfactory receptor cells of ticks. Cattle rumen volatiles were analysed after collection on a porous polymer by gas chromatography coupled with electrophysiology recordings from olfactory receptor cells in wall-pore olfactory sensilla on the first leg tarsus of Amblyomma variegatum and Ixodes ricinus. (A) Flame ionisation detector (FID) response of the gas chromatograph to the separated rumen volatiles. (B) Enlarged sections of A where butanoic (A1), isopentanoic (A2), pentanoic (A3) and hexanoic (A4) acids, 2-nitrophenol (P1), 4-methyl-2-nitrophenol (P2), 4-methylphenol (P3), indole (I1) and 3-methylindole (I2) eluted and induced electrophysiological (EL) responses from olfactory receptor cells of A. variegatum and I. ricinus (trace for A. variegatum presented). The receptor cell response profiles in B were generated by summing the frequencies of the action potentials of the responding sensory cells (frequency to voltage conversion). U indicates an unidentified stimulus. (C,D) The spike trains generated in responding (from the arrows) A. variegatum (C) and I. ricinus (D) receptor cells to 4-methylphenol, butanoic acid and indole eluting from the gas chromatographic column. Receptor cells from both tick species also responded to acetic acid, propionic acid, phenol and 2-methylphenol (data not presented). The same carboxylic acid and phenolic volatiles identified in rumen odour of cattle were also recovered in the odour of roe deer rumen. Sensillum identity (DI and DII types) is according to a classification by Hess and Vlimant (1982).
- Fig. 3.
Microbial production of volatile rumen products perceived by ticks.
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
Meet the Editors at SICB Virtual 2021
Reserve your place to join some of the journal editors, including Editor-in-Chief Craig Franklin, at our Meet the Editor session on 17 February at 2pm (EST). Don’t forget to view our SICB Subject Collection, featuring relevant JEB papers relating to some of the symposia sessions.
2020 at The Company of Biologists
Despite 2020's challenges, we were able to bring a number of long-term projects and new ventures to fruition. As we enter a new year, join us as we reflect on the triumphs of the last 12 months.
The Big Biology podcast
JEB author Christine Cooper talks to Big Biology about her research. In this fascinating JEB sponsored podcast she tells us how tough zebra finches adjust their physiology to cope with extreme climate events.
Developmental and reproductive physiology of small mammals at high altitude
Cayleih Robertson and Kathryn Wilsterman focus on high-altitude populations of the North American deer mouse in their review of the challenges and evolutionary innovations of pregnant and nursing small mammals at high altitude.
Read & Publish participation extends worldwide
“Being able to publish Open Access articles free of charge means that my article gets maximum exposure and has maximum impact, and that all my peers can read it regardless of the agreements that their universities have with publishers.”
Professor Roi Holzman (Tel Aviv University) shares his experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 60 institutions in 12 countries taking part – find out more and view our full list of participating institutions.