Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JEB
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Outstanding paper prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JEB
    • Subscriptions
    • Advertising
    • Feedback
    • For library administrators
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in
  • Log out

Search

  • Advanced search
Journal of Experimental Biology
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Experimental Biology

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JEB
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Outstanding paper prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JEB
    • Subscriptions
    • Advertising
    • Feedback
    • For library administrators
Journal Articles
Extreme resistance to desiccation in overwintering larvae of the gall fly Eurosta solidaginis (Diptera, tephritidae)
H. Ramlov, R.E. Lee
Journal of Experimental Biology 2000 203: 783-789;
H. Ramlov
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R.E. Lee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

During winter, larvae of the goldenrod gall fly Eurosta solidaginis are exposed for extended periods to severe low ambient temperatures and low humidities within plant galls. The resistance of these larvae to desiccation at various temperatures and humidities, the transition (critical) temperature, and the effects of treatment with organic solvents on the larval rates of water loss and on changes in osmolality during desiccation were examined. The water loss rates of the flesh fly Sarcophaga crassipalpis under desiccating conditions were also measured. The water permeability of the cuticle of E. solidaginis larvae was very low (0.038 microgram h(−1)cm(−2)Pa(−1) at 20 C and 4% relative humidity) compared with that of larvae of other species. The value for E. solidaginis is equivalent to that of the very drought-resistant larvae of the tenebrionid beetle Tenebrio molitor (0.038 microgram h(−1)cm(−2)Pa(−1) at 30 C). In contrast, the permeability of larvae of the flesh fly Sarcophaga crassipalpis at 20 C and 4 % relative humidity was 0.331 microgram h(−1) cm(−2)Pa(−1). The thermal dependence of the cuticular permeability increased with temperature by approximately 0.0010 +microgram h(−1) cm(−2)Pa(−1) C(−1) in the interval between 4 and 40 C. At the transition temperature of 40 C, the thermal dependence of the permeability increased abruptly to 0.0400 microgram h(−1)cm(−2)Pa(−1) C(−1). Larvae treated with hexane and acetone remained remarkably resistant to water loss. However, treatment with chloroform:methanol increased the water loss rate approximately 25-fold. During desiccation at 4 C and 4% relative humidity for 21 days, E. solidaginis larvae showed a mass loss of 18.5+/−4.4 % (mean +/− s.e.m., N=6). Animals dried under the same conditions over the same period showed a haemolymph osmolality of 851+/−75 mosmol kg(−1) (N=4). Larvae freshly removed from the galls showed a haemolymph osmolality of 918+/−67 mosmol kg(−1)(N=3). A higher osmolality in the dried compared with the fresh larvae would have been expected. The present observation suggests that important ions in the haemolymph may have been excreted or rendered osmotically inactive during desiccation.

  • © 2000 by Company of Biologists

REFERENCES

    1. Appel, A. G.,
    2. Reierson, D. A. and
    3. Rust, M. K.
    (1986). Cuticular water loss in the smokybrown cockroach Periplanata fuliginosa. J. Insect Physiol 32, 623–.
    OpenUrlCrossRef
    1. Bennett, V. A. and
    2. Lee, R. E.
    (1997). Modeling seasonal changes in intracellular freeze-tolerance of fat body cells of the gall fly, Eurosta solidaginis (Diptera: Tephritidae). J. Exp. Biol 200, 185–.
    OpenUrlAbstract/FREE Full Text
    1. Bjerke, R. and
    2. Zachariassen, K. E.
    (1997). Effects of dehydration on water content, metabolism and body fluid solutes of a carabid beetle from dry savanna in East Africa. Comp. Biochem. Physiol 118, 779–.
    OpenUrlCrossRef
    1. Collins, M. S. and
    2. Richards, A. G.
    (1963). Studies on water relations in North American termites. I. Eastern species of the genus Reticulithermes (Isoptera, Rhinotermitidae). Ecology 44, 600–.
    OpenUrl
    1. Gilby, A. R. and
    2. Rumbo, E. R.
    (1980). Water loss and respiration of Lucilia cuprina during development within the puparium. J. Insect Physiol 26, 153–.
    OpenUrlCrossRef
    1. Hadley, N. F.
    (1989). Lipid water barriers in biological systems. Prog. Lipid Res 28, 1–.
    OpenUrlCrossRefPubMedWeb of Science
    1. Hyatt, A. D. and
    2. Marshall, A. T.
    (1985). Water and ion balance in the tissues of the dehydrated cockroach, Periplaneta americana. J. Insect Physiol 31, 27–.
    1. Layne, J. R.
    (1991). Microclimate variability and the eurythermic nature of goldenrod gall fly (Eurosta solidaginis) larvae (Diptera: Tephritidae). Can. J. Zool 69, 614–.
    OpenUrl
    1. Layne, J. R.,
    2. Lee, R. E. and
    3. Huang, J. L.
    (1990). Inoculation triggers freezing at high subzero temperatures in a freeze-tolerant frog Rana sylvatica and insect Eurosta solidaginis. Can. J. Zool 68, 506–.
    OpenUrl
    1. Layne, J. R. and
    2. Medwith, R. E.
    (1997). Winter conditioning of third instars of the gall fly Eurosta solidaginis (Diptera: Tephritidae) from western Pennsylvania. Env. Ent 26, 1378–.
    OpenUrl
    1. Lee, R. E. and
    2. Denlinger, D. L.
    (1985). Cold tolerance in diapausing and non-diapausing stages of the flesh fly, Sarcophaga crassipalpis. Physiol. Ent 10, 309–.
    OpenUrl
    1. Mead-Briggs, A. R.
    (1956). The effect of temperature upon the permeability to water of arthropod cuticles. J. Exp. Biol 33, 737–.
    OpenUrlAbstract
    1. Mugnano, J. A.,
    2. Lee, R. E. and
    3. Taylor, R. T.
    (1996). Fat body cells and calcium phosphate spherules induce ice nucleation in the freeze-tolerant larvae of the gall fly Eurosta solidaginis (Diptera, Tephritidae). J. Exp. Biol 199, 465–.
    OpenUrlAbstract/FREE Full Text
    1. Storey, J. M. and
    2. Storey, K. B.
    (1988). Freeze tolerance in animals. Physiol. Rev 68, 27–.
    OpenUrlFREE Full Text
    1. Wigglesworth, V. B.
    (1945). Transpiration through the cuticle of insects. J. Exp. Biol 21, 97–.
    OpenUrlWeb of Science
    1. Wigglesworth, V. B.
    (1948). The structure and deposition of the cuticle in the adult mealworm, Tenebrio molitor L. (Coleoptera). Q. J. Microsc. Sci 89, 197–.
    OpenUrl
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Experimental Biology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Extreme resistance to desiccation in overwintering larvae of the gall fly Eurosta solidaginis (Diptera, tephritidae)
(Your Name) has sent you a message from Journal of Experimental Biology
(Your Name) thought you would like to see the Journal of Experimental Biology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Journal Articles
Extreme resistance to desiccation in overwintering larvae of the gall fly Eurosta solidaginis (Diptera, tephritidae)
H. Ramlov, R.E. Lee
Journal of Experimental Biology 2000 203: 783-789;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Journal Articles
Extreme resistance to desiccation in overwintering larvae of the gall fly Eurosta solidaginis (Diptera, tephritidae)
H. Ramlov, R.E. Lee
Journal of Experimental Biology 2000 203: 783-789;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • A maxi Cl(−) channel in cultured pavement cells from the gills of the freshwater rainbow trout Oncorhynchus mykiss
  • Three-dimensional kinematics of skeletal elements in avian prokinetic and rhynchokinetic skulls determined by Roentgen stereophotogrammetry
  • The process of cell adhesion among dissociated single cells of Hydra: morphological observations
Show more Journal Articles

Similar articles

Other journals from The Company of Biologists

Development

Journal of Cell Science

Disease Models & Mechanisms

Biology Open

Advertisement

Predicting the Future: Species Survival in a Changing World

Read our new special issue exploring the significant role of experimental biology in assessing and predicting the susceptibility or resilience of species to future, human-induced environmental change.


Big Biology Podcast - Hollie Putnam and coral bleaching

Catch the next JEB-sponsored episode of the Big Biology Podcast where Art and Marty talk to Hollie Putnam about the causes of coral bleaching and the basic biology of corals in the hope of selectively breeding corals that can better tolerate future ocean conditions.

Read Hollie's Review on the subject, which is featured in our current special issue. 


Stark trade-offs and elegant solutions in arthropod visual systems

Many elegant eye specializations that evolved in response to visual challenges continue to be discovered. A new Review by Meece et al. summarises exciting solutions evolved by insects and other arthropods in response to specific visual challenges.


Head bobbing gives pigeons a sense of perspective

Pigeons might look goofy with their head-bobbing walk, but it turns out that the ungainly head manoeuvre allows the birds to judge distance.

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Sign up for alerts

About us

  • About JEB
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists
  • Journal news

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Outstanding paper prize
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact JEB
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992