Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JEB
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Outstanding paper prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JEB
    • Subscriptions
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Journal of Experimental Biology
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Experimental Biology

  • Log in
Advanced search

RSS  Twitter  Facebook  YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JEB
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • Outstanding paper prize
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JEB
    • Subscriptions
    • Advertising
    • Feedback
Journal Articles
A Kinetic Method for the Measurement of Zinc Influx In Vivo in the Rainbow Trout, and the Effects of Waterborne Calcium on Flux Rates
D. J. SPRY, C. M. WOOD
Journal of Experimental Biology 1989 142: 425-446;
D. J. SPRY
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C. M. WOOD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & metrics
  • PDF
Loading

Summary

Three methods were evaluated to measure rate of influx of Zn into rainbow trout. The first two, disappearance of 65Zn from the water and whole-body counting, overestimated influx when compared with a third method which used a terminal plasma sample to calculate influx. The cause of the overestimate was a short-term adsorption phenomenon to both the experimental apparatus and the exterior of the fish. The third method measured only Zn which entered the fish. This method entailed ‘calibration’ of cannulated trout by constant infusion of small amounts of radiolabelled Zn. This was analogous to the entry of Zn into fish across the gill. After 24–36 h of infusion, plasma radioactivity reached a steadystate concentration which was a simple linear function of the rate of infusion. This relationship was then used to predict influx from a single terminal plasma sample from uncannulated trout exposed to radiolabelled Zn in the water. Trout acclimated to tapwater (Ca2+ = 2.0 mequivl−1) and exposed to Zn (1.5-45.9 μequivl−1; 0.05-1.5 mgl−1) showed saturable uptake which was apparently first order with no significant linear component. The apparent Jmax and Km were 314 nequiv kg−1 h−1 and 7.3 μequivl−1 (0.24 mgl−1), respectively. Acutely raising the waterborne [Ca2+] (4.7 and 9.7 mequivl−1) over the same range of [Zn] revealed a competitive type of interaction - little change in Jmax, with increased Km. When Ca2+ was acutely removed (0.05 and 1.02 mequivl−1) by the use of artificial soft water, significant linear influx occurred in addition to the saturable uptake noted at higher [Ca2+], suggesting the opening of a paracellular leak. Calculation of the inhibitor constant for Ca2+ yielded a value of 0.48 mequivl−1. This value is similar to the Km for Ca2+ when it was a transported substrate (0.28 ± 0.07 mequivl−1). The true Km for Zn transport in the absence of Ca2+ was 1.0 μequivl−1 (0.06 mgl−1). These data showed Zn influx to be saturable and strongly dependent upon waterborne [Ca2+], perhaps traversing the gill in a manner similar to Ca2+.

  • © 1989 by Company of Biologists
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Experimental Biology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
A Kinetic Method for the Measurement of Zinc Influx In Vivo in the Rainbow Trout, and the Effects of Waterborne Calcium on Flux Rates
(Your Name) has sent you a message from Journal of Experimental Biology
(Your Name) thought you would like to see the Journal of Experimental Biology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Journal Articles
A Kinetic Method for the Measurement of Zinc Influx In Vivo in the Rainbow Trout, and the Effects of Waterborne Calcium on Flux Rates
D. J. SPRY, C. M. WOOD
Journal of Experimental Biology 1989 142: 425-446;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Journal Articles
A Kinetic Method for the Measurement of Zinc Influx In Vivo in the Rainbow Trout, and the Effects of Waterborne Calcium on Flux Rates
D. J. SPRY, C. M. WOOD
Journal of Experimental Biology 1989 142: 425-446;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Info & metrics
  • PDF

Related articles

Cited by...

More in this TOC section

  • Transport of bile acids in hepatic and non-hepatic tissues
  • The anisotropic Young's modulus of equine secondary osteones and interstitial bone determined by nanoindentation
  • A novel mechanism of body mass regulation
Show more Journal Articles

Similar articles

Other journals from The Company of Biologists

Development

Journal of Cell Science

Disease Models & Mechanisms

Biology Open

Advertisement

Meet the Editors at SICB Virtual 2021

Reserve your place to join some of the journal editors, including Editor-in-Chief Craig Franklin, at our Meet the Editor session on 17 February at 2pm (EST). Don’t forget to view our SICB Subject Collection, featuring relevant JEB papers relating to some of the symposia sessions.


2020 at The Company of Biologists

Despite 2020's challenges, we were able to bring a number of long-term projects and new ventures to fruition. As we enter a new year, join us as we reflect on the triumphs of the last 12 months.


Critical temperature window sends migratory black-headed buntings on their travels

The spring rise in temperature at black-headed bunting overwintering sites is essential for triggering the physical changes that they undergo before embarking on their spring migration – read more.


Developmental and reproductive physiology of small mammals at high altitude

Cayleih Robertson and Kathryn Wilsterman focus on high-altitude populations of the North American deer mouse in their review of the challenges and evolutionary innovations of pregnant and nursing small mammals at high altitude.


Read & Publish participation extends worldwide

“Being able to publish Open Access articles free of charge means that my article gets maximum exposure and has maximum impact, and that all my peers can read it regardless of the agreements that their universities have with publishers.”

Professor Roi Holzman (Tel Aviv University) shares his experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 60 institutions in 12 countries taking part – find out more and view our full list of participating institutions.

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Sign up for alerts

About us

  • About JEB
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists
  • Journal news

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • Outstanding paper prize
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact JEB
  • Subscriptions
  • Advertising
  • Feedback

 Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992