|Copper accumulation rate (nmol mg-1 cell protein h-1)|
|Treatment||Time 0 control||After 15 min||Instantaneous Cu accumulation (%)|
|†Amiloride (2 mmol l-1)||3.00±0.38c,d||21.76±5.15*,d||13.8|
|†CDPC (10μ mol l-1)||6.39±2.01c||39.42±16.56*,d,g,e||16.2|
|†Phenamil (100 μmol l-1)||6.33±1.36c||75.39±13.47*,e||8.4|
|DIDS (100 μmol l-1)||2.79±0.33d||18.40±0.94*,f||15.1|
Values are means ± s.e.m, N=5–6 experiments.
Time 0 control; cells were pipetted into saline containing 800 μmol l-1 Cu and then immediately washed in 0.1 μmol l-1 EDTA solution.
After 15 min, Cu accumulation rate of cells at the end of a 15 min exposure to saline containing 800 μmol l-1 Cu.
% instantaneous Cu accumulation=time 0 control/after 15 min × 100%
↵* Significantly different from time 0 control (within rows) by Student's t-test or Mann–Whitney U-test (P<0.05); values within columns with one or more common superscript letters are not significantly different from each other (Kruskal–Wallis test, P>0.05)
↵† Cells were pre-incubated with the appropriate epithelial Na+ channel inhibitor for 15 min prior to the addition of Cu