Table 1.

LDH release and stability of Na+ and K+ contents in freshly isolated trout intestinal cells

Time (min)
Treatment01530
Control (no added Cu)
   Rate of LDH release<0.1<0.1<0.1
      (μmol min-1 ml-1 saline)
   Cell [Na+]11.8±1.012.3±2.313.0±4.8
      (μmol mg-1 cell protein)
   Cell [K+]1.0±0.12.0±0.31.1±0.2
      (μmol mg-1 cell protein)
Copper (800 μmol l-1)
   Rate of LDH release<0.1<0.1<0.1
      (μmol min-1 ml-1 saline)
   Cell [Na+]15.4±2.914.6±1.315.6±1.8
      (μmol mg-1 cell protein)
   Cell [K+]1.5±0.11.2±0.30.8±0.1
      (μmol mg-1 cell protein)
  • Values are means ± s.e.m., N=6 cell isolations.

    Cells were incubated in physiological saline for 30 min immediately after the cell isolation protocol.

    Control: cells in physiological saline without added Cu.

    Copper: cells from the same isolations, but incubated in saline containing the highest Cu concentration (800 μmol l-1 Cu) used in experiments.

    Rate of lactate dehydrogenase (LDH) release from cells to the medium remained below detection limit (<0.1 μmol ml-1 saline min-1) throughout in all treatments.

    No statistically significant effects of time (within rows) were observed (Kruskal–Wallis test, P>0.05) in any of the electrolyte data. No treatment effects were observed at any time point (Student's t-test, P>0.05) for cell Na+ or K+.

    Cells remain viable for at least 4 h (see text).