Table 1.

Intra-and extracellular acid-base variables of isolated hepatocytes prepared from P. brachycephalum

pHepHiPCO2 (kPa)[HCO3]i (mmol l−1)[HCO3]e (mmol l−1)
7.907.65±0.081.0210.4548.95
7.907.58±0.050.030.391.54
7.207.23±0.051.024.128.68
7.207.17±0.070.030.150.27
6.506.98±0.051.022.611.68
6.506.98±0.070.030.080.05
  • pHi values are means ± s.d., N=5-6.

    Intracellular CO2 partial pressure was assumed to equal medium PCO2.

    Apparent mean intracellular bicarbonate concentrations were calculated from mean pHi values by use of the Henderson-Hasselbach equation (see Materials and methods).