Table 1.

Pattern of drug exposure used in the experiments

Drug treatment
First synchronized preparation Second synchronized preparation
First 90 sSecond 90 sFirst 90 sSecond 90 s
First five pharynxesNo drugGramineSerotoninSerotonin+Octopamine
Second five pharynxesGramineGramine+SerotoninOctopamineOctopamine+Serotonin
Third five pharynxesSerotoninSerotonin+GramineNo drugOctopamine
  • Drug concentrations were Serotonin, 1 μmol l-1; Gramine, 100μ mol l-1; Octopamine, 100 μmol l-1.

    15 dissected pharynxes were assayed from each synchronized worm preparation.