Table 1.

Production of predominant hydrocarbons in mature D. melanogaster flies raised at various temperatures

Hydrocarbons produced (ng)
FlyHydrocarbonConstant 20°C25→20°C20→25°CConstant 25°CK—W test
Cs males7-T1043±45a1052±82a846±37b878±60a,bP<0.05
7-P106±9a112±9a225±15b154±10bP<0.001
SumCHs1584±581628±1051600±651550±87N.S.
Cs females7-T159±14a149±8a71±6b56±6bP<0.001
7-P172±12a151±8a116±7b92±7cP<0.001
7,11HD534±36a563±40a373±17b417±30a,bP<0.01
7,11ND217±16a227±28a,b295±17b306±17bP<0.05
SumCHs1986±92a,c2049±97a1724±78b1702±104b,cP<0.05
Tai males7-T264±14a290±24a74±8a,b102±7bP<0.001
7-P1002±541027±86908±62848±70N.S.
SumCHs1885±88a2014±151a1639±102a,b1540±121bP<0.05
Tai females7,11HD134±7a180±18a75±6b92±10bP<0.001
5,9HD997±44975±69817±71870±41N.S.
7,11ND168±11173±17149±15154±15N.S.
SumCHs1661±691748±1271450±1061542±92N.S.
  • Values are means of absolute amounts (ng) ± S.E.M.

    Flies of two wild-type strains, Canton-S (Cs) and Tai, were either raised at a constant temperature (20°C or 25°C) throughout development, or shifted immediately after adult eclosion from 25°C to 20°C (25→20°C) or from 20°C to 25°C (20→25°C).

    Hydrocarbons were extracted from 4- or 6-day-old flies (see Materials and methods). Hydrocarbons shown are cis 7-tricosene (7-T; 23C); cis 7-pentacosene (7-P; 25C); cis, cis7, 11-heptacosadiene (7,11HD); cis, cis5,9-heptacosadiene (5,9HD); cis, cis7,11-nonacosadiene (7,11ND), and the sum of all detected hydrocarbons (SumCHs). 5,9HD was not used for the tests because we could not separate 5,9HD and 27BR in our gas chromatograph conditions.

    For each genotype (sex and strain), the result of Kruskall—Wallis (KW) tests that were used to compare experimental groups are shown on the right. When the test was significant, a post-hoc Mann—Whitney U test was used to compare groups in pairs. Five comparisons were performed: between constant temperatures and between each constant temperature and the shift condition. (Only the two shifting conditions were not compared). Superscript letters indicate significant differences between conditions (N=8-10); N.S., not significant.