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Cover:
Electrical discontinuity of mitochondria, demonstrated by asynchronous depolarisation events induced by TMRE plus laser irradiation. Cells were loaded with the fluorescent dye TMRE, which accumulates in mitochondria due to their membrane potential. The cells were imaged using a confocal microscope with the laser set at a moderate intensity. The combination of dye plus laser irradiation results in rapid mitochondrial depolarisation events, which appear as loss of TMRE fluorescence between frames (see Collins and Bootman, pp. 1993-2000). Left panels (from top) show a TMRE-loaded pancreatic acinar cell, HeLa cell and HUVEC. The corresponding panels on the right represent maps of electrically discrete mitochondria generated by monitoring the locations of individual depolarisation events over time.
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