Article Figures & Tables
Figures
- Fig. 1.
Temperature profiles for predictable acclimation treatments over the 2-week acclimation period. Temperature measurements were taken every 5 min. Temperature data shown are from one trial, but the same acclimation treatments were repeated in all three trials. Each trial was a technical replicate. (A) Limpets in the no tide treatment were submersed in water during the entire acclimation period. (B) The predictable low treatment had low tide periods twice daily, but no heating occurred during low tide. (C) The predictable moderate treatment had low tide periods twice daily, with heating to 24°C during daytime low tide. (D) The predictable high treatment had low tide periods twice daily, with heating to 32°C during daytime low tide.
- Fig. 2.
Temperature profiles for unpredictable acclimation treatments over the 2-week acclimation period. All unpredictable acclimation treatments had two low tide periods daily, with heating only occurring during the daytime low tide. Temperature measurements were taken every 5 min. (A) Unpredictable trial 1 was taken directly from temperature data calculated as the average week at Fort Ross, CA, USA. (B,C) Unpredictable trials 2 and 3 included the same temperature peaks from unpredictable trial 1, but they were randomized in two different orders to test for the effects of stochasticity.
- Fig. 3.
Final break point temperature in heart rate for limpets and flat line temperatures from the no tide (n=29), predictable low (n=22), predictable moderate (n=25), predictable high (n=26) and unpredictable (trial 1: n=8; trial 2: n=10; trial 3: n=9) acclimation treatments. (A) Final break point temperature; (B) flat line temperature. For all predictable acclimation treatments, technical and biological replicates are grouped together. Because of statistical differences between replicate trial, each trial of the unpredictable treatment is presented separately and only biological replicates are grouped together. The line on the boxplots represents the median, the box represents the inter-quartile range (IQR), the whiskers extend 1.5 times IQR. Points beyond the whiskers are outliers. Differences in letters represent significant differences between acclimation temperatures (one-way ANOVA; Tukey’s HSD, P<0.05).
- Fig. 4.
Generalized additive mixed modeling (GAMM) for heart rates throughout the heat ramp for limpets from the no tide (n=29), predictable low (n=21), predictable moderate (n=25), predictable high (n=26) and unpredictable (trial 1: n=8; trial 2: n=10; trial 3: n=9) acclimation treatments. For all predictable acclimation treatments, technical and biological replicates are grouped together. Because of statistical differences between replicate trials, each trial of the unpredictable treatment is presented separately and only biological replicates are grouped together. Statistical differences are reported in Table 2.
- Fig. 5.
Glycogen content and citrate synthase activity in limpet foot tissue from the field (n=15) and the no tide (n=15), predictable low (n=15), predictable moderate (n=15), predictable high (n=15) and unpredictable (trial 1: n=4; trial 2: n=5; trial 3: n=5) acclimation treatments. (A) Glycogen content; (B) citrate synthase activity. Data are presented as means±s.e.m. For all predictable acclimation treatments, technical and biological replicates are grouped together. Because of statistical differences between replicate trials, each trial of the unpredictable treatment is presented separately and only biological replicates are grouped together. No statistical differences were found (one-way ANOVA). WM, wet mass
- Fig. 6.
Superoxide dismutase (SOD) activity and relative amounts of carbonylated proteins in limpet foot tissue from the field (n=15) and the no tide (n=15), predictable low (n=15), predictable moderate (n=15), predictable high (n=12 for A, n=15 for B) and unpredictable (trial 1: n=4; trial 2: n=5; trial 3: n=5) acclimation treatments. (A) SOD activity; (B) carbonylated proteins. Data are presented as means±s.e.m. For all predictable acclimation treatments, technical and biological replicates are grouped together. Because of statistical differences between replicate trials, each trial of the unpredictable treatment is presented separately and only biological replicates are grouped together. No statistical differences were found (one-way ANOVA).
Tables
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
Editors’ choice – Energetic costs of locomotion in bears
Polar bears were thought to use more energy when walking than other similarly sized animals. Now, in a unique experimental setup, Antony Pagano and co-workers measure the oxygen consumption of polar bears and grizzlies walking on a treadmill and show that they are as economic as other large animals.
Review – The effects of obesity on skeletal muscle contractile function
A new Review from Jason Tallis, Rob S. James and Frank Seebacher explores how obesity affects skeletal muscle function, thereby reducing mobility and quality of life and exacerbating the ageing process.
Featured article – Collapsing seal lungs protected by amazing anti-inflammatory blood serum
The lungs of seals might be expected to suffer damage as they collapse during deep descents and are then flooded with blood and oxygen when animals surface. This damage could also trigger a harmful inflammatory response. Yet, previous research has found no evidence that diving damages pulmonary function in seals. Now, Allyson Hindle and colleagues reveal that seal blood serum possesses anti-inflammatory properties that may protect their lungs while diving, with potential applications for the survival of organs used in life-saving transplant surgery.
Conversation – Simon Sponberg
“One of the underappreciated benefits of fellowships is the act of applying for them, because you have to write and articulate your ideas.”
In our latest early-career interview, we chat to Simon Sponberg, Assistant Professor at the Georgia Institute of Technology, USA. He shares the story of his career, beginning with how he combined a love of physics and biology by studying how geckos stick to walls.
preLights – Are Robofish accepted as a guppy conspecific?
Rasmus Ern highlights a preprint in which Jens Krause and colleagues use a magnet-controlled, 3D-printed fish replica to study social responsiveness in guppies.