The ocelli of Archaeognatha, or jumping bristletails, differ from typical insect ocelli in shape and field of view. Although the shape of the lateral ocelli is highly variable among species, most Machiloidea have sole-shaped lateral ocelli beneath the compound eyes and a median ocellus that is oriented downward. This study investigated morphological and physiological aspects of the ocelli of Machilis hrabei and Lepismachilis spp. The light-reflecting ocellar tapetum in M. hrabei is made up of xanthine nanocrystals, as demonstrated by confocal Raman spectroscopy. Pigment granules in the photoreceptor cells move behind the tapetum in the dark-adapted state. Such a vertical pigment migration in combination with a tapetum has not been described for any insect ocellus so far. The pigment migration has a dynamic range of approximately 4 log units and is maximally sensitive to green light. Adaptation from darkness to bright light lasts over an hour, which is slow compared with the radial pupil mechanism in some dragonflies and locusts.
The structure and function of compound eyes in crustaceans and insects has been the subject of intense study over the last 120 years (e.g. Exner, 1891; Horridge, 1975; Strausfeld, 2005; Warrant, 2006; Land and Nilsson, 2012); however, comparatively little is known about the ocelli (for reviews, see Goodman, 1981; Mizunami, 1995; Krapp, 2009). Adult winged insects usually have three ocelli on the frons or vertex, a median ocellus and two lateral ones – not to be confused with stemmata, which are sometimes termed ‘lateral ocelli’ as well. From a morphological point of view, they are simple eyes in that they have a single lens. Ocelli are often more sensitive than compound eyes, because of a high convergence of photoreceptors onto secondary neurons, and the fewer neurons involved in processing the signals enable a faster response. The insight that the ocelli of some taxa are capable of any resolution at all is relatively recent (Schuppe and Hengstenberg, 1993; Stange et al., 2002; Berry et al., 2007a,b); nonetheless, the compound eyes outperform ocelli in this regard. The main functional role for ocelli in winged insects is likely flight stabilization (e.g. Taylor and Krapp, 2007), an idea that can also be implemented in aerial vehicles (Gremillion et al., 2014).
But what about insects that are weak flyers or unable to fly? Most beetles, for example, have no ocelli, and the two ocelli in the cockroach Periplaneta with a 2500:1 convergence ratio from receptors to second-order neurons are obviously adapted for light sensitivity (Toh and Sagara, 1984). Among the primarily wingless basal hexapods, Protura and Diplura are blind and possess neither ocelli nor compound eyes. The condition in Collembola is somewhat unusual in that there are up to six separate groups of 2 to 12 photoreceptor cells located beneath the unmodified head cuticle and epidermis, which are considered to be homologous to insect ocelli (Paulus, 1972).
Among hexapods, well-developed ocelli only occur in the ectognathan lineage, the oldest extant representatives of which are the jumping bristletails or Archaeognatha (Misof et al., 2014). These primarily wingless insects often feed on algae and lichens are generally found in rocky and moist habitats but also on trees. The anatomy of the ocelli of Archaeognatha was first described in two species of Machilis by Hesse (1901). He mentioned a reflecting tapetum with red-brown pigment behind it, while Hanström (1940) found brown-black pigment within whole photoreceptor cells of Petrobius (Machilidae). However, neither author considered that pigment migration might account for the observed differences, nor could they resolve the nature of the tapetum, which was described as connective tissue-like. Only Wygodzinsky (1941) and Paulus (1972) independently briefly noted that the ocelli of machilids turn white during dark adaptation, but did not provide further details. In her review of insect ocelli, Goodman (1981) stated: ‘The presence of pigment granules within the retinal cells is not compatible with the use of a reflecting tapetal layer, and the two are not found in association.’ In the present paper, we will clarify these obviously conflicting statements and describe for the first time a proximal–distal ocellar pigment migration in combination with a diffusely reflecting tapetum in representatives of the Archaeognatha.
Tapeta formed by reflecting crystalline deposits are quite common among animals, e.g. fish, cats, mollusks, spiders, crustaceans and insects (for a review, see Schwab et al., 2002). Although crystals of various substances can form multi-layer reflectors, the best studied is guanine (Land, 1972; Mueller and Labhart, 2010; Hirsch et al., 2015; Bossard et al., 2016; Jordan et al., 2014, 2012). Guanine or uric acid reflectors also cause color and color change phenomena in the integument of many animals, e.g. copepods (Chae et al., 1996), beetles (Caveney, 1971), spiders (Insausti and Casas, 2008) and chameleons (Teyssier et al., 2015).
The determination of the chemical nature of multilayer reflectors has been performed by chromatography, UV-Vis spectroscopy and histochemistry (Zyznar and Nicol, 1971; Chae et al., 1996; Mueller and Labhart, 2010). In the present analysis we demonstrate that Raman microspectrometry is likewise an appropriate method that even has considerable advantages over the others.
In Raman spectroscopy, the sample is illuminated with monochromatic light. A small fraction of the scattered light shows shifted frequencies because of interaction with vibrational modes characteristic of the sample (Raman and Krishnan, 1928). The benefits of confocal Raman spectroscopy in identifying the chemical nature of the tapetum crystals are twofold: the method is easy to use (simply place the sample on a slide) and the possible sub-micrometer spot size enables acquisition of spectra from very small sample areas. In our case, this is important because the tapetum cannot be mechanically separated from the pigment containing photoreceptor cells. Using a confocal Raman microscope, a suitable region can easily be targeted.
The goals of the present paper are twofold. First, to report our findings on the functional morphology of the ocelli in representatives of Archaeognatha. The results include a detailed description of the proximal–distal pigment migration in the photoreceptor cells that was found in all examined species. Second, to elucidate the chemical nature of the tapetum, its nanocrystals, their dimensions and arrangement. In addition, the data are discussed in light of several speculations on the biological role of the ocelli.
MATERIALS AND METHODS
Machilis hrabei Kratochvil 1945, Lepismachilis y-signata Kratochvil 1945 and Lepismachilis notata Stach 1919 were collected in the surroundings of Vienna, Austria, and kept indoors under the naturally prevailing light/dark cycle. Acheta domesticus (Linnaeus 1758) were bought in a local pet shop and stored frozen until they were dissected.
Standard histological techniques were employed (see Böhm et al., 2012) with the only exception that transmission electron microscopy (TEM) sections were contrasted with 2.5% gadolinium triacetate (Nakakoshi et al., 2011) instead of uranyl acetate. To examine single tapetum crystals, the dissected tissue was placed on the surface of distilled water. Mechanical stress and probably osmotic effects instantly cause the crystals to disperse out of the ruptured tissue. This allows them to be collected with Formvar-coated TEM grids. A Zeiss 902 or Libra 120 TEM was used to image the specimens.
Focal length determination
The lens of the median ocellus was dissected from the specimen in phosphate buffered saline and examined with a Leitz Dialux microscope without condenser using the ‘hanging drop’ method (Homann, 1924).
Ocelli were dissected in deionized water and dried on an aluminum-coated slide. Raman spectra were acquired using a Horiba LabRAM HR Evolution confocal microspectrometer (Horiba Scientific, Edison, NJ, USA) with a 532 nm laser and 300 or 600 mm−1 gratings. Spectra were baselined with the program Spekwin (version 1.72.0, http://www.effemm2.de/spekwin).
Pigment migration and spectral sensitivity
To record light adaptation dynamics, a dark-adapted animal (40 min) was placed under a Nikon SMZ 25 stereomicroscope and illuminated with bright white light (40 kLux). The computer program Fiji (Schindelin et al., 2012) was used to plot the mean pixel value of the ocelli in the recorded images against time.
In the other experiments, a Leica S6 E stereomicroscope was used, which was equipped with an 850 nm IR LED (infrared light emitting diode, ILH-IO01-85SL-SC201-WIR200) mounted onto one ocular and a camera mounted to the other ocular (MotiCam 2300; IR blocking filter replaced by an IR transparent longpass filter). Images were acquired with a custom μManager (https://www.micro-manager.org) script and data were plotted with LiveGraph (http://www.live-graph.org). LEDs driven by a custom constant current power supply were used as light sources (peak wavelengths: 365 nm: NCSU275T-U365; 460±10 nm: LXML-PB01-0023; 530±10 nm: LXML-PM01-0090; 590±5 nm: LXML-PL01-0060; 630±10 nm: LXML-PH01-0060). The light output from the LEDs was measured by placing a calibrated photodiode (FDS100, Thorlabs, Munich, Germany) under the microscope instead of the animal. The whole setup was shielded from ambient light and experiments were performed at 17°C between 18:00 h and 23:00 h.
To measure the spectral sensitivity, the intensity of the respective LED was adjusted until the same steady-state response (a certain mean pixel value) was achieved. The inverse of this intensity value is considered the sensitivity. Measurements were made from short to long wavelengths and in the reverse order to check for adaptation effects.
In M. hrabei, the lateral ocelli are tilted 55 deg outward with respect to the sagittal plane and are oriented slightly upward with the median third being tilted outward only 20 deg. Hence the overall shape is concave and the median third covers a more lateral part of the visual field than the remaining ocellus. The median ocellus is located on an anterior prominence of the head capsule at the edge between the frons and clypeus (Fig. 1). Its orientation is very unusual in that it is directed downward at an angle of approximately 65 deg (between the optical axis and a horizontal section plane; Fig. 1B). Moreover, the field of view is narrowed by the large maxillary palps and only a triangular window is left open by the shape and usual position of these palps. The cuticle of the lateral ocelli is only slightly curved and thus can be considered a transparent window (Fig. 2A,B), while the cuticle of the median ocellus is somewhat more lens-like (Figs 1E and 2C). Underneath the cuticle is a single-layered epidermis with small irregularly shaped nuclei (Fig. 2D). Slender processes from these epidermal cells extend between the photoreceptor cells. Each of the mostly X-shaped rhabdoms is formed by four photoreceptor cells with large, round nuclei that are located distally (Fig. 3A). Axons of the photoreceptor cells extend a short distance medially from the lateral ocelli, where they form a globular neuropil, adjacent to the brain, with dendrites of ‘downstream’ neurons (Hanström, 1940). Proximally, the photoreceptor cells run through holes within a tapetum consisting of cells with large elongated nuclei that are densely packed with xanthine crystals (Fig. 3C,E). One lateral ocellus has 105±13 (n=3) such holes in its tapetum, which equals approximately 420 photoreceptor cells per ocellus because the photoreceptor cells run through the holes in groups of four (Fig. 3B).
Optics of the ocelli
Many of the concepts used to describe the sensitivity and resolution of animal eyes assume a circular aperture and an image that is focused on the retina (Land, 1981). As these two assumptions are clearly violated in the lateral ocelli, we focused our attention on the optical properties of the median ocellus of M. hrabei. The back focal distance (i.e. the distance from the back surface of the lens to the image) of dissected median ocellus lenses measured by the hanging drop method was 203±67 μm (n=4). The rather high variation is attributed to different body size of the animals and to the difficulty of orienting the optical axis of the dissected lens perfectly parallel to that of the microscope.
The reflection spectrum of the tapetum or the amount of specular versus diffuse reflection has yet to be measured. When visually examining dark-adapted ocelli that are illuminated by incident light from various angles, the reflection seems to be mainly diffuse. However, the partly ordered tapetum suggests that the reflection could have diffuse and specular components. In the following optical model, we assume full specular reflection, as that is the only case that can be readily calculated and, while not observed so far, it could occur naturally in species with a slightly more ordered crystal arrangement. In addition, we qualitatively discuss the effect of a diffuse reflection.
The position of the focal point of the lens–mirror system (Fig. 4) was determined using the ‘vergence method’ of geometrical optics (e.g. Meyer-Arendt, 1995). The initial convergence of a distant point source, C0, is zero and the subsequent convergences Ci were found by considering the optical powers of the refracting (P0,P1) and reflecting (P2) interfaces of radii rj and adding terms for the distances tk between them: (1)
The distance of the focal point from the back surface of the lens was t1–(n2/C5)=45 μm and, after adding half the thickness of the lens, this can be taken as approximation of the focal length f=54 μm of a single thin lens that has the same effect as the calculated combination (values used in the calculation are as follows, where n are refractive indices, lengths in μm: n0=1.0, n1=1.5, n2=1.33, t0=18, t1=65, r0=57, r1=100 and r2=–50). The back focal distance of the lens alone is given by n2/C3=164 μm. Under the simplifying assumption of a circular aperture with diameter A=100 μm, this gives a very low F number of f/A=0.54. The rhabdoms have a cross-sectional area of 37.9±23.3 μm2 (n=53, ratio of rhabdom to retinal area pr=0.17) which corresponds to a circle with diameter d=6.9 μm. For the rhabdomere absorption coefficient k, the value obtained for lobster (Warrant and Nilsson, 1998) is assumed, and the rhabdomere length l is 40 μm. Now the sensitivity of the ocellus for an extended scene illuminated by white light can be estimated as (Warrant and Nilsson, 1998). In this formula, stands for the fraction of light absorbed by photopigments within the rhabdoms. This value can be corrected, accounting for the fact that rhabdoms only cover a fraction of the retina, by multiplication with pr. In the dark-adapted state, light that is not absorbed in the first pass is reflected back through the retina. This effect can be approximated by multiplication of S by pr+pr(1–P)=pr(2–P). According to Speiser and Johnsen (2008), this correction factor would only be 1–P because they assume that the absorption of unfocused light in the first pass decreases sensitivity. We suggest that the light absorbed in the first pass through the retina, which is pre-focused by the cuticular lens, increases sensitivity while decreasing resolution. So the final sensitivities in the light- and dark-adapted states become Slight=pr S=1.8 μm2 sr and Sdark=pr(2–P)S=3.4 μm2 sr, respectively.
The back focal distance of the slightly curved surface of the lateral ocellus calculated as above was 831 μm (r0=200 μm, r1=207 μm, other parameters as for the median ocellus). This is approximately 15 times the depth of the lateral ocellus, thus the refracting interface behaves essentially like a flat light window. The curvature in the tapetum is convex in the central region, while in the median and lateral parts it is concave (Fig. 2A,B).
Tapetum crystal arrangement
The individual crystals of the tapetum are small rhomboid platelets of 440±111 nm edge length and an obtuse angle of 99.1±4.8 deg as measured from free crystals on a Formvar film (n=20; Fig. 3D,F). Although the solubility of xanthine in water (0.069 g l−1 at 16°C O'Neil, 2006) and alcohol is low, the crystals are almost always lost during ultrathin section preparation. In favorable sections with at least partially remaining crystals, the resulting gaps are informative. These gaps are 56±24 nm (n=78) wide and 363±122 nm (n=122) long. The crystals often occur in groups of two crystals that have the same orientation and a smaller distance between them (70±19 nm, n=38) than between two adjacent crystals that are not necessarily from the same ‘package’ (100±50 nm, n=20). These packages, as well as the individual crystals, are enclosed by a membrane and typically two such packages are stacked in a tapetum cell in the direction of incident light (Fig. 3E). The crystals are preferentially orientated with their largest face parallel to the cuticle of the ocelli, and the standard deviation of the angle at which the crystals are oriented in the tapetum was determined to be 22 deg (from seven horizontal sections). The thickness of the tapetum lies between 12 to 30 μm and consists of a stack of 20 to 30 crystal layers.
Confocal Raman spectroscopy
Raman microspectroscopy of the tapetum of M. hrabei yielded spectra (Fig. 5A) with a close correspondence of peak positions and intensities to those of xanthine (http://www.sigmaaldrich.com/spectra/rair/RAIR000286.PDF, #X7375). For comparison, the white tapetum material in the ocelli of Acheta domesticus, which contain no pigment, was likewise examined with Raman spectroscopy (Fig. 5B). The spectrum agrees with that of uric acid (http://www.sigmaaldrich.com/spectra/rair/RAIR002210.PDF, #U2625), although not as well as in the xanthine case. The red pigment of M. hrabei ocelli has a spectrum with three poorly separated peaks at 1330, 1487 and 1625 cm−1 (Fig. 6).
Dynamics and spectral sensitivity of pigment migration
In light-adapted M. hrabei, large amounts of red pigment granules in the retinula cells are located in front of the tapetum but migrate completely behind it upon dark adaptation (Fig. 2A,B,D). The tapetum is visible as a white border around the lateral ocellus in light-adapted M. hrabei but not in the investigated species of Lepismachilis.
The mechanism that triggers the pigment migration is maximally sensitive to green light (Fig. 7A). Because of the limited number of LED wavelengths and comparatively high spectral half-width, the wavelength of peak sensitivity can only be determined as 530±30 nm. The sensitivity curve falls off more steeply on the long-wavelength side of the peak than in the blue–UV region.
The pigment migration response is not ‘all or nothing’ but reaches a steady state corresponding to the irradiance of the ocellus. For the lateral ocellus of M. hrabei, this response follows a sigmoid function (Fig. 7B) and the dynamic range spans approximately four decades. The temporal dynamics of the transition from the dark- to the light-adapted state likewise resulted in a sigmoid curve when plotting the reflectance of the ocellus over time after switching on the light (Fig. 7C–E, Movie 1). Full adaptation to bright light requires approximately 1.5 h and is comparably fast in M. hrabei and Lepismachilis notata.
Sensitivity and resolution
A striking feature in machilids is the oddly shaped lateral ocelli. While sole-shaped lateral ocelli are probably a plesiomorphic character for Machiloidea, various other shapes and positions occur (e.g. Dilta: round, lateral; Trigoniophthalmus: tear shaped, median; Machilinus: round, lateral) (Sturm and Machida, 2001). With our present knowledge on the biology of Archaeognatha, it is not possible to clearly correlate the shape of the lateral ocelli with any particular lifestyle, but a reasonable assumption is that the ocellus area is enlarged to enhance photon capture in dark environments.
The only potential for resolution in the lateral ocelli can be derived from the curvature of the lateral ocellus, because light incident from lateral (yellow arrows in Fig. 1E) will produce higher irradiance in the median part, whereas frontal light will lead to higher irradiance in the lateral part. The acceptance angle of a rhabdom, , depends on the refractive indices of the rhabdom, nrh, and of the outer medium, no (Miller, 1979). This will, together with shading effects, enhance resolution of the lateral ocelli in the light-adapted state because screening pigment raises no, an effect observed in various compound eyes (Miller, 1979). However, this increase in resolution will be rather small given the weak ocellus lens, and we hypothesize that the main function of the pigment migration is to attenuate intense daylight.
Most Archaeognatha have black, brown or red pigment in their ocelli; only some Meinertellidae, especially Machilinus sp., seemingly lack any pigment (Sturm and Machida, 2001; Sturm and Smith, 1993). Remarkably, Machilinus species are active during the day (Sturm, 1997), in contrast to almost all other Archaeognatha. The presence of black ocelli in members of the subgenus Protomachilinus could be indicative of an adaptation toward activity in a darker environment (Sturm, 1997). Insects that face strongly changing light intensities during their activity periods probably need a way to protect their sensitive photoreceptors from overexposure. Other insects that are active under more constant illumination conditions, such as Periplaneta (darkness) and Locusta (diurnal), often lack ocellar pigment and their ocellar photocreceptors could be adapted to these specific light intensities.
The median ocellus could have some spatial resolution in the dark-adapted state if the reflection from the tapetum were specular. Nonetheless, the incident and reflected light would first pass through the ‘wrong’ rhabdoms until it finally reached the focal point. This would be a detrimental condition in terms of resolution, as noted for other eyes with reflectors by Land and Nilsson (2012). The sensitivity of the median ocellus clearly lies above the typical values for apposition compound eyes in Archaeognatha. For comparison, the compound eye of a bee has a calculated sensitivity (Land, 1981) that is approximately 10 times lower than the estimate we obtained for the dark-adapted median ocellus of M. hrabei.
To summarize, we hypothesize that the ocelli of Archaeognatha have reasonable sensitivity, permitting crepuscular activity, yet very poor resolution. The lateral ocelli have a larger field of view and a potential for some resolution because of their shape. Mizunami (1995) suggested that the ancestral insect ocellar system (e.g. Odonata) had both bi- and trisynaptic pathways and was concerned with optimization of both speed and sensitivity. Given the condition found in Archaeognatha [fast jumping/gliding behaviors (see below), adjustable sensitivity], we anticipate that such a neural organization could also be present in Archaeognatha.
In the past few years, theoretical and experimental interest in guanine multilayer reflectors was very high because of potential biomimetic applications (Bossard et al., 2016; Iwasaka et al., 2016; Hirsch et al., 2015; del Barco et al., 2015; Gur et al., 2015a,b, 2013; Jordan et al., 2014, 2012; Oaki et al., 2012; Levy-Lior et al., 2008). These recent theoretical works have taken disorder into account, i.e. variation in the thickness of crystal and inter-crystal layers; however, in more diffuse reflectors, the three degrees of rotational freedom of the crystals needs to be addressed as well.
No clear-cut evolutionary pattern of tapetum types in hexapod ocelli is discernible. Reflective crystal tapeta are relatively common in insect ocelli, for example, in cicadas (Ribi and Zeil, 2015), dobsonflies (Megaloptera), odonates, cockroaches and locusts (Goodman, 1981). Tracheolar tapeta in the ocelli are reported to occur in Diptera (Goodman, 1970), Hymenoptera (Somanathan et al., 2009) and tentatively also Trichoptera (Hallberg and Hagberg, 1986). Thus, despite the present scarcity of data, the presence of tracheolar tapeta in ocelli could be an evolutionary novelty of certain Holometabola. In the hemimetabolous Odonata, tracheoles between the photoreceptor cells seem to optically insulate them from each other to some degree (Stange et al., 2002).
The reflective tapetum of crustacean nauplius eyes is in some groups crystalline, e.g. in ostracods (Meyer-Rochow, 1999; Andersson and Nilsson, 1981) and copepods (Fahrenbach, 1964), yet in ‘Conchostraca’, round granules occur that are comparable to those in crustacean compound eyes (Reimann and Richter, 2007). It may be hypothesized that vesicles filled with high refractive index substances are an evolutionary precursor to crystalline tapeta because the crystals also form inside membranous vesicles (Fig. 3E).
Tapetum crystal and screening pigment chemistry
Several reviews and textbooks claim that the tapetum is formed of urate crystals in Schistocerca (Goodman, 1970), Periplaneta (Goodman, 1981) and in the ‘typical insect ocellus’ (Chapman, 1998; Lancaster and Downes, 2013; Klowden, 2013). However, as rigorous tests demonstrating the chemical nature of the tapetum crystals have rarely been performed, our finding of xanthine, which is very similar to both guanine and uric acid, might not be uncommon. In crustaceans, high amounts of xanthine were found in mantis shrimp eye extract, but it was not determined whether it occurs specifically in the tapetum (Zyznar and Nicol, 1971). In the same study, 19 malacostracan eyes were examined for purines and pteridines, but the reflecting layers were separately analyzed only in Penaeus setiferus. In this species, the main component found was isoxanthopterin, and it was concluded that it could cause the observed reflection. In Homarus, reflective layers in the compound eye contain uric acid, xanthine, hypoxanthine, xanthopterin and an unidentified pteridine, but it is unclear which causes the reflection (Kleinholz, 1959). Kleinholz (1959) also found that the reflecting layer in Limulus eyes mainly contains guanine.
Xanthine is known to be created either from guanine by guanine deaminase, from hypoxanthine by xanthine oxidoreductase or from xanthosine by purine nucleoside phosphorylase, and it is converted to urate by xanthine oxidase (KEGG pathway ko:00230; Kanehisa et al., 2014). Some lichens inhibit xanthine oxidase (Behera et al., 2003), which might cause elevated xanthine levels in animals consuming them. Although this has only been reported for tropical lichen species, it may bear some significance as the investigated Archeaognatha were fed with lichens. The same 100 deg angle as in archaeognathan xanthine crystals was found in the monoclinic unit cell of the tetrahydrate sodium salt of xanthine (Mizuno et al., 1969). It is thus plausible that the flat face of the crystals, which is oriented toward the incident light, is the (010) face of monoclinic crystals. Nonetheless, further investigation, e.g. using electron diffraction, is needed to verify this observation because the crystals are also compatible with the triclinic or orthorhombic systems.
The Raman spectrum of the red pigment (Fig. 6) encountered in the ocelli of M. hrabei is similar to the unidentified blue pigment spectra in chameleons (Teyssier et al., 2015) and the red and green pigment spectra in lizards (Saenko et al., 2013). They, in turn, resemble the Raman spectra of xanthopterin and isoxanthopterin (Saenko et al., 2013; Feng et al., 2001). There is little correspondence with the Raman spectra of melanin, carotene and the simulated spectrum of the ommochrome ommin (Hsiung et al., 2015).
Migration of pigment, in the shape of a pupil formed by specialized cells between the lens and the receptor cells, has been described for the ocelli of dragonflies (Stavenga et al., 1979) and locusts (Wilson, 1975). Goodman (1981) also lists bees as having such an iris, but does not provide further details. In Heteroptera, vertical migration of pigment occurs in the photoreceptor cells of the tapetum-less ocelli of Rhodnius (Goodman, 1981) and Triatoma (Insausti and Lazzari, 2002). In Odonata, the retinula cells are ensheathed by tapetal cells whose nuclei are located distally, whereas the nuclei of the retinula cells are situated proximally above a layer of pigment cells (Ruck and Edwards, 1964). The retinula cells do not contain pigment granules as observed in Archaeognatha.
The spectral sensitivity (i.e. the action spectrum) of the pigment migration in ocelli has, to our knowledge, only been determined for two species of Odonata (Stavenga et al., 1979). Regarding compound eyes in several insects (Stavenga, 1979) and the crustaceans Procambarus (Olivo and Chrismer, 1980) and Gonodactylus (Cronin and King, 1989), it was shown that the action spectrum of the pigment migration closely matches the photoreceptor response spectrum. Only putative long-wavelength-sensitive opsin was found to be expressed in head regions containing ocelli of various odonates in a transcriptome sequencing study (Futahashi et al., 2015). Interestingly, the response to UV stimuli was twofold to ninefold higher than to green stimuli in the dragonfly Hemicordulia tau (van Kleef et al., 2005). In locusts, Wilson (1978) found evidence for UV and green visual pigment.
Apart from light, temperature is another factor that profoundly influences pigment migration in compound eyes of insects (Nordström and Warrant, 2000) and crustaceans (King and Cronin, 1994a,b). In the latter, microtubules are believed to mediate the granule transport (King and Cronin, 1994b). In the bug Triatoma, neither temperature nor circadian rhythm has an effect on the pigment position inside the ocellar retinula cells (Lazzari et al., 2011). It seems that pigment migration in the pigment cells of insect compound eyes is not neuronally controlled but rather is modulated by catecholamines (Hamdorf et al., 1989). Wilson (1975) found that the adaptation mechanism for the radial pupil in some locusts clearly depends neither on the circadian rhythm nor on neural or hormonal control. It is not known whether these pupil cells are photosensitive themselves or are electrically or chemically influenced by the nearby photoreceptor cells.
Functional role of the ocelli
Among arthropods, and especially insects, the ocelli of Archaeognatha are unique in that the visual field of their median ocellus is oriented toward the ground. A possible function of the median ocellus could be to sense the optical properties of the surface the animals walk upon, in order to select a spot that provides optimal camouflage. Blending with their environment is obviously vital for Archaeognatha as most of them have a cryptic coloration over the entire body, including the compound eyes. The median ocellus could also be useful for spatial orientation because it faces the sky when the animals crawl upside down on tree limbs or peak out from the undersides of rock slabs, where they often rest.
Ocelli apparently only play a minor role in circadian entrainment (Saunders, 1976; Rieger et al., 2003; Tomioka and Abdelsalam, 2004), although nothing is known about this topic in Archaeognatha. Likewise, there is no information on whether the ocelli in Archaeognatha are involved with navigational behaviors, as in ants (Schwarz et al., 2011a,b).
Polarization sensitivity has been reported for few hymenopteran ocelli (for a review, see Zeil et al., 2014). It requires that the microvilli are parallel and the rhabdomere is as straight as possible (Ribi et al., 2011). In the X-shaped rhabdomes of archaeognathan ocelli, parts of two almost perpendicular arms are formed by each of the four receptor cells (Fig. S1). Thus no or very low net polarization sensitivity can be expected (Waterman, 1981).
The main function of well-developed ocelli in winged insects is flight stabilization (Wilson, 1978; Mizunami, 1995; Krapp, 2009). Although Archaeognatha cannot fly, they jump (Evans, 1975), and some of them achieve a directed aerial descent from trees (Yanoviak et al., 2009). When dropped from a tree, they glide back to the trunk, which most likely indicates a visually guided behavior. The relative importance of the compound eyes versus the ocelli has not been investigated in this behavior so far. Dudley and Yanoviak (2011) proposed an eight-step scenario for the evolution of flight, the first five of which Archaeognatha fulfill. Steps one to three are residence on elevated substrates, jumping/falling and aerial righting/landing reflexes. Therefore, it is possible that the ocelli already started to play an important role during the aerial endeavors of the primarily wingless Archaeognatha by providing faster feedback and higher sensitivity in low light conditions compared with compound eyes. It would be a highly interesting topic for future studies to determine the relative contribution of the ocelli and the compound eyes to the jumping and gliding behaviors of Archaeognatha.
We thank Markus Schmid (Department of Microbial Ecology, University of Vienna) for enabling the Raman measurements, the Core Facility Cell Imaging and Ultrastructure Research (University of Vienna) for access to the TEM, John Plant for checking the English and two anonymous reviewers for their constructive comments.
The authors declare no competing or financial interests.
A.B. conceived the study, conducted the experiments and drafted the manuscript. G.P. contributed to the manuscript.
The Austrian Science Fund FWF provided financial support (P 23251-B17).
Supplementary information available online at http://jeb.biologists.org/lookup/doi/10.1242/jeb.141275.supplemental
- Received March 31, 2016.
- Accepted July 18, 2016.
- © 2016. Published by The Company of Biologists Ltd