Mammalian hair cells possess only a limited ability to repair damage after trauma. In contrast, sea anemones show a marked capability to repair damaged hair bundles by means of secreted repair proteins (RPs). Previously, it was found that recovery of traumatized hair cells in blind cavefish was enhanced by anemone-derived RPs; therefore, the ability of anemone RPs to assist recovery of damaged hair cells in mammals was tested here. After a 1 h incubation in RP-enriched culture media, uptake of FM1-43 by experimentally traumatized murine cochlear hair cells was restored to levels comparable to those exhibited by healthy controls. In addition, RP-treated explants had significantly more normally structured hair bundles than time-matched traumatized control explants. Collectively, these results indicate that anemone-derived RPs assist in restoring normal function and structure of experimentally traumatized hair cells of the mouse cochlea.
The authors declare no competing or financial interests.
All authors had full access to all data in the study and take responsibility for the integrity of the data. P.-C.T. performed experiments, and acquired and analyzed the data. P.-C.T. and G.M.W. designed the study, interpreted the data and were involved in manuscript preparation. K.M.S. assisted in culturing explants of the organ of Corti.
This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.
Supplementary information available online at http://jeb.biologists.org/lookup/doi/10.1242/jeb.135459.supplemental
- Received November 30, 2015.
- Accepted May 18, 2016.
- © 2016. Published by The Company of Biologists Ltd