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Journal of Experimental Biology partnership with Dryad

Aging and its modulation in a long-lived worker caste of the honey bee
Daniel Münch, Claus D. Kreibich, Gro V. Amdam

SUMMARY

Highly social animals provide alternative aging models in which vastly different lifespan patterns are flexible, and linked to social caste. Research in these species aims to reveal how environment, including social cues, can shape the transition between short-lived and extremely long-lived phenotypes with negligible senescence. Among honey bee workers, short to intermediate lifespans are typical for summer castes, while the winter caste can live up to 10 times longer. For summer castes, experimental interventions could predictably accelerate, slow or revert functional senescence. In contrast, little is known about the partic ular conditions under which periods of negligible senescence in winter castes can be disrupted or sustained. We asked how manipulation of social environment in colonies with long-lived winter bees might alter the pace of functional senescence, measured as learning performance, as well as of cellular senescence, measured as lipofuscin accumulation. We show that behavioral senescence becomes rapidly detectable when the winter state is disrupted, and changes in social task behaviors and social environment (brood) are induced. Likewise, we found that cellular senescence was induced by such social intervention. However, cellular senescence showed marked regional differences, suggesting that particular brain regions age slower than others. Finally, by preventing post-winter colonies from brood rearing, behavioral senescence became undetectable, even after transition to the usually short-lived phenotypes had occurred. We envision that social regulation of negligible functional senescence and highly dynamic accumulation of a universal symptom of cellular aging (lipofuscin) offers rewarding perspectives to target proximate mechanisms of slowed aging.

FOOTNOTES

  • AUTHOR CONTRIBUTIONS

    D.M., G.V.A. and C.D.K. conceived and designed the experiments. D.M. and C.D.K. performed the experiments. D.M. and G.V.A. analyzed the data. D.M. drafted the manuscript. G.V.A. and D.M. revised the manuscript.

  • Supplementary material available online at http://jeb.biologists.org/cgi/content/full/216/9/1638/DC1

  • COMPETING INTERESTS

    No competing interests declared.

  • FUNDING

    G.V.A. and D.M. were supported by the Norwegian Research Council (180504, 191699, 213976/F20); G.V.A. received support from the PEW Charitable Trust, the National Institute on Aging (PO1AG22500).

  • LIST OF ABBREVIATIONS

    CS
    conditioned stimulus
    GRS
    gustatory responsiveness
    HP
    hypopharyngeal
    KW
    Kruskal–Wallis analysis of variance
    LS
    learning score
    MWU
    Mann–Whitney U-test
    PER
    proboscis extension response
    US
    unconditioned stimulus
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