JEB desktop wallpaper calendar 2016

JEB desktop wallpaper calendar 2016

Sperm metabolism in pigs: a role for peroxisome proliferator-activated receptor gamma (PPARγ)
M. Santoro, C. Guido, F. De Amicis, D. Sisci, D. Vizza, S. Gervasi, A. Carpino, S. Aquila

SUMMARY

Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear hormone receptor expressed predominantly in adipose tissue, also implicated in energy homeostasis. In this study, we used western blotting and immunofluorescence techniques to demonstrate for the first time that pig spermatozoa express PPARγ. To investigate the functional role of PPARγ in pig sperm, we evaluated its action on different events that characterize the biology of sperm cells, i.e. motility, capacitation, viability and acrosome reaction, using the PPARγ-agonist 15-deoxy-12,14-prostaglandin J2 (PGJ2). In responses to PGJ2 treatment, motility, cholesterol efflux and tyrosine phosphorylation were increased, which broadens the role of PPARγ from that previously described in the literature, as it also acts to improve sperm functionality. To further our understanding of the significance of PPARγ in pig sperm, we focused its effects on lipid and glucose metabolism. Evaluation of triglyceride content and lipase, acyl-CoA dehydrogenase and G6PDH activities suggests that PPARγ induces energy expenditure in pig spermatozoa. These data represent a meaningful advance in the field of sperm energy metabolism. Taken together, our results demonstrate for the first time that PPARγ is expressed by pig sperm, thus improving its functionalities in terms of motility, capacitation, acrosome reaction, survival and metabolism.

FOOTNOTES

  • * These authors contributed equally to this work

  • Joint senior authors

  • FUNDING

    This work was supported by Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR) Ex 60% (2012).

  • LIST OF ABBREVIATIONS

    Ab
    antibody
    AKT
    RAC-PK-alpha
    BBS
    balanced buffered solution
    BCL2
    B-cell lymphoma 2
    BSA
    bovine serum albumin
    CHOD–POD
    cholesterol-oxidase–peroxidase
    DGGR
    1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin)ester
    ECL
    enhanced chemiluminescence
    FITC
    fluorescein isothiocyanate
    G6PDH
    glucose-6-phosphate dehydrogenase
    GW
    irreversible PPARγ antagonist GW9662
    MAPK
    mitogen-activated protein kinase
    NC
    uncapacitated sample without treatment
    PBS
    phosphate buffered saline
    PGJ2
    15-deoxy-12,14-prostaglandin J2
    PI
    propidium iodide
    PI3K
    phosphoinositide 3-kinase
    PNA
    peanut agglutinin
    PPAR
    peroxisome proliferator-activated receptor
    PPP
    pentose phosphate pathway
    TBS
    Tris-buffered saline
  • View Full Text