Electrogenic proton-regulated oxalate/chloride exchange by lobster hepatopancreatic brush-border membrane vesicles

SUMMARY The transport of [14C]oxalate (Ox2–) by epithelial brush-border membrane vesicles (BBMV) of lobster (Homarus americanus) hepatopancreas, formed by a magnesium precipitation technique, was stimulated by an outward Cl– gradient (in > out). By contrast, Ox2– uptake was not enhanced by an inward Na+ or K+ transmembrane gradient. Generation of an inside-positive membrane potential by K+ in the presence of valinomycin stimulated Ox2–/Cl– exchange, while an inside-negative membrane potential generated by K+ efflux in the presence of valinomycin inhibited this process. Neither Ox2–/Ox2– nor Ox2–/SO42– transport exchange were affected by alterations of transmembrane potential. An inwardly directed proton gradient, or the presence of low bilateral pH, enhanced Ox2–/Cl– exchange, yet the H+ gradient alone could not stimulate Ox2– uptake in Cl–-equilibrated BBMV or in vesicles lacking internal Cl–. The stilbenes 4-acetamido-4′-isothiocyanotostilbene-2,2′-disulfonic acid (SITS) and 4,4′-diisothiocyano-2,2′-disulfonic stilbene (DIDS) strongly inhibited Ox2–/Cl– exchange. Oxalate influx occurred by a combination of carrier-mediated transfer, exhibiting Michaelis–Menten kinetics, and nonsaturable `apparent diffusion'. Apparent kinetic constants for Ox2–/Cl– exchange were Kt=0.20 mmol l–1 and Jmax=1.03 nmol l–1 mg–1 protein 7 s–1. 36Cl– influx into oxalate-loaded BBMV was stimulated by an inside-negative transmembrane potential compared with short-circuited vesicles. These results suggest that Ox2–/Cl– exchange in crustacean hepatopancreatic BBMV occurred by an electrogenic carrier mechanism exhibiting a 1:1 flux ratio that was modulated by an external proton-sensitive regulatory site.

In invertebrates, specifically crustaceans, the hepatopancreas is involved in both digestion and absorption of nutrients (Gibson and Barker, 1979); in addition, some investigators have implicated this organ as a site of excretion (Dall, 1970). The use of isolated membrane vesicles has led to the definition of absorptive transport mechanisms for a number of solutes in the hepatopancreatic brush border (Ahearn and Clay, 1987a,b;Ahearn et al., 1985;Gerencser et al., 1996) and also for a number of transport processes, including those for Ox 2-, in the basolateral membrane (Gerencser et al., 1995(Gerencser et al., , 2000. The present study uses hepatopancreatic brush-border membrane vesicles (BBMV) to characterize an electrogenic Ox 2-/Clexchange mechanism that is relatively specific with respect to organic anions, is inhibited by stilbenes and is regulated by pH. The mechanism is reversible and, therefore, it can be utilized for either absorption or secretion of Ox 2-(for excretory purposes) from the lobster hepatopancreatic epithelium.

Materials and methods
Live Atlantic lobsters (Homarus americanus L.; 0.5·kg each) were purchased from commercial dealers in Florida and Hawaii and maintained unfed at 10°C for up to 1·week in filtered seawater. All animals were either in intermolt or early premolt as assessed by the molt stage classification scheme introduced by Aiken (1973).
Hepatopancreatic brush-border membrane vesicles (BBMV) were prepared from fresh tissue removed from individual lobsters. Each membrane batch was produced from a single organ (15-25·g fresh mass) using a method of combined osmotic disruption, differential centrifugation and magnesium precipitation described previously by Ahearn and co-workers (Ahearn and Clay, 1987a;Ahearn et al., 1985). Purity of BBMV prepared by these methods was assessed by comparing the activities of membrane-bound enzymes in vesicles with the activities of the same enzymes in the original homogenate. These comparisons showed final pellet enrichments of alkaline phosphatase (marker enzyme for apical membranes), Na + /K + -ATPase (marker enzyme for basolateral membranes) and cytochrome c oxidase (marker enzyme for mitochondrial membranes) of 19.3-, 0.7-and 0.2-fold, respectively, suggesting that the isolated vesicles were highly enriched with apical membranes and contained minimal contamination from basolateral or organelle membranes.
Transport studies were conducted at 15°C using the rapid filtration technique developed by Hopfer et al. (1973). For time-course experiments, a volume of vesicles (e.g. 20·µl) was added to a volume of incubation media (e.g. 180·µl) containing 0.1·mmol·l -1 radiolabeled [ 14 C]oxalate (specific activity 12.2·µCi·mmol -1 ). At various incubation times, a known volume (20·µl) of reaction mixture was removed and plunged into 2·ml of ice-cold stop solution (stop solution composition varied with experiment and generally consisted of incubation media without any oxalate to stop the uptake process). The vesicle suspension was then rapidly filtered through 0.65·µm Millipore filters (presoaked in distilled water) and washed with another 5·ml of ice-cold stop solution. Filters were transferred to vials containing Ready Solv HP scintillation cocktail (Beckman, Chicago, IL, USA) and counted for radioactivity in a Beckman LS-8100 scintillation counter. Transport experiments involving incubations of less than 10·s were conducted using a rapid-exposure uptake apparatus (Inovativ Labor AG, Adliswil, Switzerland). Uptake was initiated by mixing 5·µl of vesicles with a volume (e.g. 45·µl) of radiolabeled incubation media, and filters were washed and counted for radioactivity as above. For short-term incubations, a blank was also run for each condition by mixing stop solution, vesicles and radiolabeled incubation media simultaneously; the resulting value was subtracted from corresponding experimental results before determining uptake. Incubation and intravesicular media varied between experiments and are indicated in the figure legends. Oxalate uptake values were usually expressed as pmol·mg -1 ·protein (Bio-Rad protein assay) per filter using the specific activity of oxalate in the incubation media.
Unless otherwise indicated in the text or in the figure legends, valinomycin (50·µmol·l -1 ) and bilaterally equal potassium concentrations (10-100·mmol·l -1 ) across the vesicular wall were present to short-circuit the membranes. Each experiment was repeated at least four times using membranes prepared from different animals. Within a given experiment each point was determined from three to five replicate samples and the experimental scatter between these replicates never exceeded 5%. Differences between experimental and control means were analyzed using a Student's t-test. Results of experiments are presented as means ± S.E.M.
Oxalate uptake into BBMV was not stimulated by incubation in media containing 100·mmol·l -1 K-gluconate (no valinomycin or internal K + ) nor in the presence of 50·µmol·l -1 CCCP (a protonophase used to short-circuit the membrane potential in the absence of K + and valinomycin, which are the normal reagents used to short-circuit the membrane potential; Fig.·3). However, when vesicles were preloaded with 25·mmol·l -1 KCl and incubated in TMA-gluconate media containing equimolar K + and valinomycin, there was an overshoot Ox 2accumulation approximately twice that of the equilibrium value. Such results, and those from Figs·1,·2, indicate an anion exchange mechanism that can utilize a Clgradient, but not a gradient of HCO3 -, as a driving force to move Ox 2across the apical membrane.

Specificity of the oxalate-exchange process
The questions of whether Ox 2would exchange with a wide range of organic ions as transferable substrates was investigated by trans-stimulation with either 5·mmol·l -1 oxalate, sulfate, oxaloacetate, succinate, formate, αketoglutarate or citrate (Fig.·5). Only oxalate and sulfate were able to stimulate 0.1·mmol·l -1 [ 14 C]Ox 2uptake greater than the response obtained in the presence of the nonexchangable anion gluconate. This experiment indicates that in the lobster hepatopancreatic BBMV the antiporter is relatively specific and will not accept similar dicarboxylic acids or the tricarboxylic acid citrate as a substrate.

Effects of pH on oxalate-chloride exchange
Oxalate uptake has been shown to be stimulated by proton (or hydroxyl) gradients in the basolateral membrane (BLM) of the teleost fish renal epithelium (Renfro and Pritchard, 1982). The possible proton (hydroxyl) gradient stimulation of Ox 2-/Clexchange in hepatopancreatic BBMV was investigated in a series of experiments in which the pH of internal and external media was varied and 0.1·mmol·l -1 [ 14 C]Ox 2uptake was determined. Fig.·6 shows that when the pH of the incubation media was maintained at 7.0 and the internal pH was varied from pH 5.0 to 8.0 there was an inequality of Ox 2transport into the BBMV preloaded with 100·mmol·l -1 Cl -. The lowest internal pH of 5.0 corresponded to the slowest uptake of Ox 2-, whereas when the internal pH was greater (pHin=8.0) than the external pH, Ox 2uptake was enhanced over that of all other conditions. Alternatively, the observed phenomena may have been attributed to competition of internal hydroxyl concentration with Cl -.
In a reciprocal experiment where the internal pH was kept constant at pH 7.0 and the pH of the incubation media was raised from 5.0 to 8.0, there was again a gradation of Ox 2uptake into vesicles preloaded with 100·mmol·l -1 Cl - (Fig.·7). With a decrease in external pH there was a corresponding increase in Ox 2accumulation. These results indicate that an increase in external proton (or decrease in external hydroxyl) concentration can stimulate Ox 2-/Clexchange.
In contrast to the previous two experiments, Fig.·8 shows the result of Ox 2-/Clexchange at various bilateral pH conditions ranging from 5.0 to 8.0. The maximal uptake of Ox 2into BBMV occurred at pH 5.0 and decreased as pH was raised to 8.0. Results of this experiment indicate that it was not the proton (or hydroxyl) gradient that stimulated Ox 2uptake but rather the external absolute proton (hydroxyl) concentration.
In the present study, extravesicular Na + or K + gradients caused no intravesicular accumulation of Ox 2- (Fig.·1), which rules out cotransport of Ox 2with either of these two cations. An outward vesicular gradient of HCO3had no accumulative effect on Ox 2into the BBMV, which negates an Ox 2-/HCO3antiporter as a possible mechanism for Ox 2transport (Fig.·1). Ruling out the possibility that Ox 2transport across the brushborder membrane occurs via a Clchannel, as has been shown and speculated in other species (Freel et al., 1998;Hatch et al., 1984Hatch et al., , 1994, we demonstrated that 9-AC, a known Clchannel blocker (Freel et al., 1998), had no effect on the downhill energetic movement of Ox 2into the BBMV of lobster hepatopancreatic epithelium (Fig.·2).
The effect of valinomycin-induced K + diffusion potentials on Ox 2-/Clexchange was investigated to determine the effect of membrane potential on Ox 2transport in the BBMV of P=0.17 nmol mg -1 protein 7 s -1 mmol l -1 Jmax=1.03 nmol mg -1 protein 7 s -1 Kt=0.20 mmol l -1 Oxalate concentration (mmol l -1 ) Oxalate uptake (nmol mg -1 protein 7 s -1 ) Fig.·11. Effect of external Ox 2concentration on the uptake of 0.1·mmol·l -1 [ 14 C]Ox 2into hepatopancreatic BBMV. Vesicles were preloaded with 100·mmol·l -1 TMA-Cl, 50·mmol·l -1 K-gluconate and 50·µmol·l -1 valinomycin. Incubation media contained 100·mmol·l -1 TMA-gluconate, 50·mmol·l -1 K-gluconate and indicated oxalate concentrations. All media contained 40·mmol·l -1 Hepes-Tris at pH 7.0. lobster hepatopancreatic epithelium. Oxalate uptake was measured under both inside-negative and inside-positive vesicular membrane potential conditions and compared with short-circuited conditions. Fig.·4 indicates that Ox 2-/Clexchange is stimulated by a positive vesicular interior while inhibited by a negative vesicular interior. These data suggest that there is an excess of negative charge transferred into the vesicle during the exchange process. These data also support the idea that the carrier can accommodate one oxalate ion and one chloride ion, which would result in an electrogenic Ox 2-/Clexchange, as has been demonstrated for SO4 2-/Clexchange in the same epithelium (Gerencser et al., 1995).
In the lobster hepatopancreas in vivo there is a pH gradient maintained across the epithelium, with a lower pH in the lumen than in the blood (Gibson and Barker, 1979). We therefore tested the effect of pH gradients on the uptake of Ox 2into the BBMV. Ox 2-/Clexchange was diminished when the internal pH was less than the external pH (Fig.·6), and Ox 2uptake was enhanced as the external pH was decreased (constant internal pH), as shown in Fig.·7. This suggested that an extravesicular pH lower than internal pH could stimulate Ox 2-/Cl -(or Ox 2-/OH -) exchange. When the pH was held constant on both sides of the BBMV (Fig.·8), there was increased uptake at lower pH, an effect similar to that obtained in Fig.·6. This result suggested that it was not the pH gradient but the lower external pH (increased external protons) that stimulated Ox 2-/Clexchange.
This series of experiments suggests that a proton (or hydroxyl) gradient does not act as a driving force during Ox 2-/Clexchange, nor could it stimulate uptake alone, yet there were significant effects of varying pH on the magnitude of Ox 2-/Clexchange. All of the experiments were shortcircuited by the presence of valinomycin and equimolar K + across the BBMV so that H + -generated transmembrane diffusion potentials would be unlikely to cause the results observed in Figs·6 and 7. Protons have been shown to act as allosteric activators of the Na + /H + exchanger in rabbit renal BBMV (Aronson et al., 1982) and Na + /SO4 2cotransport in rabbit ileum BBMV (Ahearn and Murer, 1984). Similarly, the results observed in Figs·6-8 would support the idea of external protons having a modifier role for stimulating the Ox 2-/Clexchanger.
The existence of internal pH-sensitive regulatory sites for the Cl -/HCO3exchanger has been demonstrated on the rabbit ileal brush-border membrane using membrane vesicles (Mugharbil et al., 1990), in isolated cell preparations of lymphocytes (Mason et al., 1989) and in Vero cells (Olsnes et al., 1986). An external pH-sensitive regulatory site on the Ox 2-/Clexchanger in the hepatopancreatic brush-border membrane would be physiologically important due to the 2Na + /H + exchanger also present in the membrane (Ahearn et al., 1990). The 2Na + /H + exchanger operates during luminal acidification following ingestion of a meal and would provide a stimulus for enhanced Ox 2-/Clexchange. When the 2Na + /H + exchanger is operating there would be an increase in the proton concentration in the hepatopancreatic lumen and a corresponding decrease of protons in the cytoplasm. This would tend to enhance the Ox 2-/Clexchanger due to modification at an external site, as demonstrated in Fig.·8.
The influx of [ 14 C]oxalate in hepatopancreatic BBMV occurred by at least one carrier-mediated mechanism exhibiting Michaelis-Menten kinetics and a second process that may be simple diffusion (Fig.·11). The apparent affinity constant (Kt) for oxalate binding to the vesicle exterior was 0.20·mmol·l -1 , and the maximal transport velocity (Jmax) was 1.03·nmol·mg -1 ·protein·7·s -1 . The apparent diffusional component had a rate of 0.31·nmol·mg -1 protein·7·s -1 ·mmol·l -1 . These kinetic constants are similar to those obtained for oxalate transport across the BLMV of lobster hepatopancreas (Gerencser et al., 1995(Gerencser et al., , 2000. The Ox 2-/Clexchanger was significantly (P<0.05) inhibited by the disulfonic stilbenes DIDS and SITS, as shown in Fig.·10. Oxalate transport has been shown to be inhibited by SITS and DIDS in other epithelial preparations (Pritchard, 1987;Renfro et al., 1987;Renfro and Pritchard, 1983). Furosemide and bumetanide were not effective as inhibitors in the present study (Fig.·10). The strong inhibition by DIDS and SITS provides further evidence for the presence of an anion exchanger in hepatopancreatic BBMV since both SITS and DIDS are relatively specific inhibitors for anion exchange processes (Renfro and Pritchard, 1983;Shiu-Ming and Aronson, 1988;Talor et al., 1987).
Under the assumption that exchange mechanisms such as this can operate in both directions, it should be possible to measure the uptake of 36 Clinto vesicles in exchange for internal Ox 2-. If the exchange operated at a 1:1 ratio it would be expected to react to a membrane potential. It was observed that 36 Clinflux did respond to an inside-negative potential (Fig.·9) by an increased uptake during its incubation. With this arrangement there would be a secretion of Ox 2from the vesicular interior in exchange for luminal Clthat is further driven by the inside negative potential that is characteristic of epithelial cells (Gerencser, 1985). This secretion of Ox 2would lead to its excretion unless it were recycled by the same antiporter. This phenomenon would support the work of some investigators who have postulated that the crustacean gut can provide an excretory function in the elimination of certain solutes (Dall, 1970;Gifford, 1962).
The brush-border membrane Ox 2-/Clexchanger described in the present investigation cannot provide definitive evidence for absorption or secretion for either Ox 2or Cl -. However, speculatively, this mechanism could provide a cellular means for ridding the cell of Ox 2and/or taking up Clfor metabolic functions. This transport would allow for the elimination of Ox 2from the lobster hepatopancreatic cells as a means of anion regulation. Oxalate binds divalent cations, such as Ca 2+ , and precipitates as calcium oxalate, which could be deleterious for cellular survival (Binder, 1974;Dobbins and Binder, 1977;Earnest, 1974). However, a more realistic function of Ox 2may be that of storing Ca 2+ as calcium oxalate in hepatopancreatic lysosomes and mitochondria for the use of Ca 2+ during the molt cycle, as has been previously reported (Gerencser et al., 1996(Gerencser et al., , 2000. The anion antiporter could then be used for ridding the cell of excessive Ox 2as a cytoprotective mechanism after completion of the molting cycle.