QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online September 15, 2004
Journal of Experimental Biology 207, 3649-3656 (2004)
Published by The Company of Biologists 2004
doi: 10.1242/jeb.01219

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JEB Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Buckley, B. A. Articles by Hofmann, G. E. Search for Related Content PubMed PubMed Citation Articles by Buckley, B. A. Articles by Hofmann, G. E.

Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish, Trematomus bernacchii

Bradley A. Buckley^*, Sean P. Place and Gretchen E. Hofmann

Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93106-9610, USA

Author for correspondence (e-mail: hofmann{at}lifesci.ucsb.edu)

Accepted 2 August 2004

The Antarctic fishes, isolated over evolutionary history in the sub-zero waters of the Southern Ocean, are an ideal group for studying the processes of cold adaptation. One species of Antarctic notothenioid fish, Trematomus bernacchii, has lost the ability to induce heat shock proteins (Hsps) in response to exposure to acute thermal stress or to the toxic heavy metal cadmium, an important part of the cellular defense response to such stressors. To elucidate the mechanism responsible for the lack of Hsp induction, we examined several stages of the hsp gene expression pathway, including transcription factor activity, Hsp70 mRNA production and protein synthesis patterns, in hepatocytes from T. bernacchii. Hsp70 mRNA was detected, as was heat shock factor 1 (HSF1) with DNA-binding activity. However, exposure to elevated temperature and to chemical inducers of the heat shock response failed to increase Hsp70 mRNA levels, HSF1 activity or the concentration of any size class of Hsps. These results suggest that Hsps, inducible in nearly every other species, are expressed constitutively in the cold-adapted T. bernacchii.

Key words: heat shock protein, Hsp70, hepatocyte, constitutive expression, Trematomus bernacchii, Notothenioid, Antarctica

Related articles in JEB:

HEAT SHOCK GETS A CHILL

Kathryn Phillips
JEB 2004 207: i. [Full Text]  

This article has been cited by other articles:

				Z. Chen, C.-H. C. Cheng, J. Zhang, L. Cao, L. Chen, L. Zhou, Y. Jin, H. Ye, C. Deng, Z. Dai, et al. Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish PNAS, September 2, 2008; 105(35): 12944 - 12949. [Abstract] [Full Text] [PDF]

				K. P. P. Fraser, A. Clarke, and L. S. Peck Growth in the slow lane: protein metabolism in the Antarctic limpet Nacella concinna (Strebel 1908) J. Exp. Biol., August 1, 2007; 210(15): 2691 - 2699. [Abstract] [Full Text] [PDF]

				J. P. Rinehart, A. Li, G. D. Yocum, R. M. Robich, S. A. L. Hayward, and D. L. Denlinger Inaugural Article: Up-regulation of heat shock proteins is essential for cold survival during insect diapause PNAS, July 3, 2007; 104(27): 11130 - 11137. [Abstract] [Full Text] [PDF]

				J. P. Rinehart, S. A. L. Hayward, M. A. Elnitsky, L. H. Sandro, R. E. Lee Jr, and D. L. Denlinger Continuous up-regulation of heat shock proteins in larvae, but not adults, of a polar insect PNAS, September 19, 2006; 103(38): 14223 - 14227. [Abstract] [Full Text] [PDF]

				G. E. Hofmann Patterns of Hsp gene expression in ectothermic marine organisms on small to large biogeographic scales Integr. Comp. Biol., April 1, 2005; 45(2): 247 - 255. [Abstract] [Full Text] [PDF]

				K. Phillips HEAT SHOCK GETS A CHILL J. Exp. Biol., October 1, 2004; 207(21): i - i. [Full Text] [PDF]