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BANTA, L. M., ROBINSON, J. S., KLIONSKY, D. J. AND EMR, S. D. (1988). Organelle assembly in yeast: Characterization of yeast mutants defective in vacuolar biogenesis and protein sorting. J. Cell Biol. 107, 1369Ð1383. BOWMAN, E. J., SIEBERS, A. AND ALTENDORF, K. (1988). Bafilomycins: a new class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc. natn. Acad. Sci. U.S.A. 85, 7972Ð7976. FOURY, F. (1990). The 31-kDa polypeptide is an essential subunit of the vacuolar ATPase in Saccharomyces cerevisiae. J. biol. Chem. 265, 18554Ð18560. HIRATA, R., OHSUMI, Y., NAKANO, A., KAWASAKI, H., SUZUKI, K. AND ANRAKU, Y. (1990). Molecular structure of a gene, VMA1, encoding the catalytic subunit of H+-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. biol. Chem. 265, 6726Ð6733. JOHNSON, L. M., BANKAITIS, V. A. AND EMR, S. D. (1987). Distinct sequence determinants direct intracellular sorting and modification of a yeast vacuolar protein. Cell 48, 875Ð885. 92 D. J. KLIONSKY AND OTHERS KLIONSKY, D. J., BANTA, L. M. AND EMR, S. D. (1988). Intracellular sorting and processing of a yeast vacuolar hydrolase: Proteinase A propeptide contains vacuolar targeting information. Molec. cell. Biol. 8, 2105Ð2116. KLIONSKY, D. J. AND EMR, S. D. (1989). Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase. EMBO J. 8, 2241Ð2250. KLIONSKY, D. J. AND EMR, S. D. (1990). A new class of lysosomal/vacuolar protein sorting signals. J. biol. Chem. 265, 5349Ð5352. KLIONSKY, D. J., HERMAN, P. K. AND EMR, S. D.(1990). The fungal vacuole: composition, function, and biogenesis. Microbiol. Rev. 54, 266Ð292. KLIONSKY, D. J., NELSON, H. AND NELSON, N.(1992). Compartment acidification is required for efficient sorting of proteins to the vacuole in Saccharomyces cerevisiae. J. biol. Chem. 267, 3416Ð3422. KORNFELD, S. AND MELLMAN, I.(1989). The biogenesis of lysosomes. A Rev. Cell Biol. 5, 483Ð525. MORIYAMA, Y. AND NELSON, N. (1989). H+-translocating ATPase in Golgi apparatus. Characterization as vacuolar H+-ATPase and its subunit structures. J. biol. Chem. 264, 18445Ð18450. NELSON, H., MANDIYAN, S. AND NELSON, N. (1989). A conserved gene encoding the 57-kDa subunit of the yeast vacuolar H+-ATPase. J. biol. Chem. 264, 1775Ð1778. NELSON, H. AND NELSON, N. (1989). The progenitor of ATP synthases was closely related to the current vacuolar H+-ATPase. FEBS Lett. 247, 147Ð153. ROBINSON, J. S., KLIONSKY, D. J., BANTA, L. M. AND EMR, S. D. (1988). Protein sorting in Saccharomyces cerevisiae: Isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Molec. cell. Biol. 8, 4936Ð4948. ROTHMAN, J. H., HUNTER, C. P., VALLS, L. A. AND STEVENS, T. H. (1986). Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene. Proc. natn. Acad. Sci. U.S.A. 83, 3248Ð3252. SHIH, C.-K., WAGNER, R., FEINSTEIN, S., KANIK-ENNULAT, C. AND NEFF, N. (1988). A dominant trifluoperazine resistance gene from Saccharomyces cerevisiae has homology with FoF1 ATP synthase and confers calcium-sensitive growth. Molec. cell. Biol. 8, 3094Ð3103. STEVENS, T. H., ROTHMAN, J. H., PAYNE, G. S. AND SCHEKMAN, R. (1986). Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y. J. Cell Biol. 102, 1551Ð1557. WILSON, D. W., WILCOX, C. A., F LYNN, G. C., C HEN, E., KUANG, W.-J., HENZEL, W. J., BLOCK, M. R., ULRICH, A. AND ROTHMAN, J. E. (1989). A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature 339, 355Ð359. WINTHER, J. R., STEVENS, T. H. AND KIELLAND-BRANDT, M. C. (1991). Yeast carboxypeptidase Y requires glycosylation for efficient intracellular transport, but not for vacuolar sorting, in vivo stability, or activity. Eur. J. Biochem. 197, 681Ð689. YAMASHIRO, C. T., KANE, P. M., WOLCZYK, D. F., PRESTON, R. A. AND STEVENS, T. H. (1990). Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Molec. cell. Biol. 10, 3737Ð3749.


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