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RUNHAAR, G., SCHEDLER, J. AND MANLEY, G. A. (1991). The potassium concentration in the cochlear fluids of the embryonic and post-hatching chick. Hearing Res. 56, 227Ð238. RUSSELL, V. E. W., KLEIN, U., REUVENI, M., SPAETH, D. D., WOLFERSBERGER, M. G. AND HARVEY, W. R. 354 U. KLEIN (1992). Antibodies to mammalian and plant vacuolar-type ATPase cross react with the vacuolar-type ATPase of insect cation-transporting plasma membranes. J. exp. Biol. 166, 131Ð143. SCHWEIKL, H., KLEIN, U., SCHINDLBECK, M. AND WIECZOREK, H. (1989). A vacuolar-type ATPase, partially purified from potassium-transporting plasma membranes of tobacco hornworm midgut. J. biol. Chem. 264, 11136Ð11142. STRANGE, K. AND PHILLIPS, J. E. (1985). Cellular mechanism of HCO32 and Cl2 transport in insect salt gland. J. Membr. Biol. 83, 25Ð37. THOMSON, R. B. AND PHILLIPS, J. E. (1992). Electrogenic proton secretion in the hindgut of the desert locust, Schistocercagregaria. J. Membr. Biol. 125, 133Ð154. THURM, U. AND WESSEL, G. (1979). Metabolism-dependent transepithelial potential differences at epidermal receptors of arthropods. J. comp. Physiol. 134, 119Ð130. WELTENS, R., L EYSSENS, A., ZHANG, S. L., LOHRMANN, E., S TEELS, P. AND VAN KERKHOVE, E. (1992). Unmasking of the apical electrogenic H+ pump in isolated Malpighian tubules (Formica polyctena) by the use of barium. Cell Physiol. Biochem. 40 (in press). WESSING, A., HEVERT, F. AND RÖNNAU, K. (1987). Ion transport and intracellular activity of ions in Malpighian tubules of Drosophilahydei. Zool. Beitr. N.F. 30, 297Ð314. WIECZOREK, H. (1982). A biochemical approach to the electrogenic potassium pump of insect sensilla: potassium sensitive ATPase in the labellum of flies. J. comp. Physiol. 148, 303Ð311. WIECZOREK, H. (1992). The insect V-ATPase, a plasma membrane proton pump energizing secondary active transport: molecular analysis of electrogenic potassium transport in the tobacco hornworm midgut. J. exp. Biol. 172, 335Ð343. WIECZOREK, H. AND GNATZY, W. (1985). The electrogenic potassium pump of insect cuticular sensilla. Further characterization of ouabain-and azide-insensitive K+-stimulated ATPase in the labellum of the blowfly. Insect Biochem. 15, 225Ð232. WIECZOREK, H., PUTZENLECHNER, M., ZEISKE, W. AND KLEIN, U. (1991). A vacuolar-type proton pump energizes H+/K+-antiport in an animal plasma membrane. J. biol. Chem. 266, 15340Ð15347. WIECZOREK, H., WOLFERSBERGER, M. G., CIOFFI, M. AND HARVEY, W. (1986). Cation-stimulated ATPase activity in purified plasma membranes from tobacco hornworm midgut. Biochim. biophys. Acta 857, 271Ð281. WIGGINS, P. M., VAN RYN, R. T. AND ORMROD, D. G. C. (1991). Donnan membrane equilibrium is not directly applicable to distributions of ions and water in gels or cells. Biophys. J. 60, 8Ð14. WIGGLESWORTH, V. B. (1948). The insect as a medium for the study of physiology. Proc. R. Soc., Lond. 23, 430Ð446. WOLFERSBERGER, M. G. (1992). V-ATPase-energized epithelia and biological insect control. J. exp. Biol. 172, 377Ð386. ZEISKE, W. (1992). Insect ion homoestasis. J. exp. Biol. 172, 323Ð334. V-ATPase in insect ion-transporting epithelia jeb8526 fig. 1 tip-in Fig. 1. Apical localization of plasma membrane V-ATPase in insect ion-transporting epithelia: midgut, Malpighian tubules and sensilla. Light microscopy, about 10mm cryosections, immunofluorescence by monoclonal antibodies to the midgut V-ATPase and FITC-conjugated secondary antibodies (for details of methods, see Klein et al. 1991; Klein and Zimmermann, 1991). (A) Posterior; (B) middle midgut of larval Manduca sexta, labelling of the goblet cell apical membrane; (C) Malpighian tubule of larval M. sexta; labelling of the apical brush border; (D) antennal sensillum coeloconicum of adult Antheraea pernyi; labelling of the goblet cell apical membrane. Note the strong autofluorescence of the cuticle. a, apical; b, basal. Scale bars, 10mm.
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