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Files in this Data Supplement:
Fig. S1. Larval zebrafish that received a capped mRNA injection expressed strong green fluorescence at 1 dpf (A) and the fluorescence was evidently reduced as a result of the degradation of the injected capped mRNA at 2 dpf (B). No fluorescence was observed in the larval zebrafish that received capped mRNA and antisense atp2b1a morpholinos (C,E 1 dpf; D,F, 2 dpf). Control fish at 2 dpf without any injection are shown in G and H for comparison. Scale bars, 200 µm.
Fig. S2. Whole-body calcium influx and calcium content of zebrafish larvae. There are no significant differences between the atp2b1a morphants (MT, injected with 6 ng of atp2b1a antisense morpholinos), and wild-type (WT) larvae. The vertical bar represents one standard deviation.
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