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Files in this Data Supplement:
Fig. S1. Separation and purification of the rPO-CHH in E. coli. (A) 18% SDS-PAGE, lane 1, insoluble pellets; lane 2, supernatant. M=protein molecular weight marker. The molecular weights are listed on the left hand side of gel. Arrows indicate the position of rPO-CHH. (B) Elution profile of RP-HPLC. Purification of the rPO-CHH in the supernatant on C18 Gemini column connected with RP-HPLC. The absorbance was monitored at 210 (solid line) and 254 nm (dotted line). The peak (*) was collected manually and was examined for cross-reactivity with anti-PO-CHH serum by a dot blot analysis and for molecular mass determination by MADLI-TOF (8447.4 Da). The linear line shows the gradient of solvent B. Solvent A: 0.11% (w/v) TFA in water, solvent B: 0.1% (w/v) TFA in 60% acetonitrile and 40% water.
Fig. S2. Displacement curve of 125I-ES-CHH (closed circle) and 125I-PO-CHH (closed square) with scaphognathites membrane. 100,000 DPM (∼47 fmol) of 125I-ES-CHH (closed circle) or 125I-PO-CHH was incubated for 1 h with 100 µg of scaphagonathite membranes in the presence of homologous neuropeptide concentration ranging from 5E−12 to 5E−7 mol l−1.
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