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Fig. 1. (A) Primary structure of the urea transporter (UT) isolated from the kidney
of the red-eared slider turtle, Trachemys scripta elegans. The
deduced amino acid sequence is aligned with those of rat UT-A2 (U09957) and
rat UT-B2 (U81518) using the Clustal algorithm. Asterisks denote identical
amino acid residues to turtle UT. The horizontal bars indicate the predicted
transmembrane regions. The box indicates putative N-glycosylation
sites (NIT). The ALE domain, which is considered to be a signature sequence
for the UT-B, is underlined. (B) Kyte–Doolittle hydropathy profile of
the deduced Turtle UT amino acid sequence predicts the presence of
transmembrane regions (1–10). (C) Phylogenetic tree showing the
relationship between vertebrate UTs. The tree was constructed by the
neighbor-joining method using ClustalW based on UT sequences. Numbers at
branch points are derived from bootstrap analysis (1000 repetitions). UTs of
bacteria, Desulfovibrio and Ochrobactrum, were regarded as
outgroups. Scale bar represents a phylogenetic distance of 0.1 amino acid
substitutions per site. The position of turtle UT is boxed. Each sequence
appears in the protein database with the following accession nos:
Desulfovibrio UT,YP-010379; Ochrobactrum UT, YP-001370983;
fugu (pufferfish) UT-C, NP001033079; eel UT-C, BAD66672; human UT-B, AAH50539;
mouse UT-B, AAI00571; rat UT-B, EDL84685; Triakis UT, BAC75980;
stingray UT, AAQ23382; eel eUT, BAC53976; toadfish UT, AAD53976; tilapia UT,
AAG49891; fugu UT, NP001027896; toad UT, BAF16706; frog UT, CAA73322; turtle
UT, AB308450; rat UT-A2, AAA84392; whale UT-A2, BAF46914; and human UT-A2,
CAA65657.
