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Fig. 2. Receptor cells in taste buds of juvenile trout. (A) A receptor cell from a
taste bud in the vomer, stained with L-cysteine (100 mmol
l–1) + FM1-43 (1 µmol l–1). The taste bud
was exposed for 30 s and fixed with paraformaldehyde in 0.1 mol
l–1 phosphate buffer. (B) A taste bud on the tongue with
three sensory cells stained with L-cysteine (100 mmol
l–1) + FM1-43 (1 µmol l–1; 5 min). The
images are z-projections taken using a fluorescence microscope with 2.5 µm
distance, stack parameter `max intensity' and processed with ImageJ. The scale
bar is for both images.