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Fig. 2. Densities and distribution of DHPRs and RyRs in the skin of coeval adult
female (non-layers) and male chickens on a low-Ca2+ diet. (A, B)
Fluorescence intensities in different skin layers after incubation with
high-affinity (–)-enantiomers of DHP and ryanodine, respectively. (C)
Distribution of DHPRs in the skin of one female, non-laying chicken. (D)
Distribution of DHPRs in the skin of one coeval male chicken. (E) Distribution
of RyRs in the skin of one female, non-laying chicken. (F) Distribution of
RyRs in the skin of one male chicken. Error bars denote s.e.m.; ST-SC,
innermost SC and the interface between the ST and SC; EPI, mid-epidermis; D,
outermost dermis. *P<0.05, post hoc tests.
Bar, 50 µm.